297692.fig.005a
(a)
297692.fig.005b
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297692.fig.005c
(c)
Figure 5: Cellular effects of TN122 on the proliferation and viability of Kasumi-1 cells. Incubation of Kasumi-1 cells for 7 days in X-Vivo 10 medium with daily cotreatment of the cells with 8 μM TN122 or TBCR as a control protein and 5 μM TAT-HA2. (a) Western blot detection of both cell-penetrating proteins in the cellular lysates 5 hours after the last addition of the proteins. (b) Analysis of the proliferation rates of treated Kasumi-1 cells. At day 0, 2 × 105 c/mL were seeded, and cell numbers were measured daily by trypan blue staining. The values are mean values with the corresponding standard deviation of the experiment carried out in triplicates. Data were statistically analyzed using two-tailed student’s t test for unpaired samples; was considered significant (*) and highly significant (**). (c) Analysis of the percentage of apoptotic cells by flow cytometry at day 7. Shown is the percentage of cells that are double positive for Annexin V and 7AAD. The values are mean values with the corresponding standard deviation of the experiment carried out in duplicates.