Research Article

Antigenicity and Immunogenicity of Rotavirus VP6 Protein Expressed on the Surface of Lactococcus lactis

Figure 4

Humoral immune response in mice immunized with L. lactis NZ9000/pCWA:VP6, NZ9000, or PBS. (a) Anti-VP6 IgG levels in mice sera collected at days 0, 14, 28, and 43 after first inoculation as determined by ELISA. Bars represent mean OD490 and error bars represent SEM for each group. Mouse polyclonal antibodies anti-RRV rotavirus was used as a positive control. (b) Western blot analysis of VP6-specific IgG in mice sera collected at day 43 after first inoculation with NZ9000/pCWA:VP6 (lanes 1 to 5), NZ9000 plasmid-free (lane 6), or PBS (lane 7). Representative membranes strips individually probed with sera diluted 1/100 are shown. Mouse polyclonal antibodies anti-RRV rotavirus was used as a positive control (+). The sizes of molecular weight standards (MWS) (in kilodaltons) and the size of the VP6 band are denoted on the left and right of the membrane, respectively.
298598.fig.004a
(a)
298598.fig.004b
(b)