Membrane Localization of Membrane Type 1 Matrix Metalloproteinase by CD44 Regulates the Activation of Pro-Matrix Metalloproteinase 9 in Osteoclasts

Figure 7

Reducing the levels of CD44, MT1-MMP, and MMP9 attenuates OC migration but not polarization, sealing ring formation, and bone resorption. Confocal microscopy analysis of distribution of actin in indicated osteoclasts cultured on dentine slices. Osteoclasts were stained for actin (red; (a–d)). Resorption pits underneath the osteoclasts are shown separately in (a′)–(d′). Dentine slices are shown in green by the reflected light. Experiments were repeated two times. Three dentine slices were used for each condition in both experiments. Results represent one of the two experiments and dentine slices performed with two different osteoclast preparations. Scale bar: 25 μm.