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BioMed Research International
Volume 2013 (2013), Article ID 307602, 6 pages
http://dx.doi.org/10.1155/2013/307602
Research Article

Preparation, Modification, and Characterization of Alginate Hydrogel with Nano-/Microfibers: A New Perspective for Tissue Engineering

1Nucleus of Cellular and Tecidual Biology (NCTBio), Post-Graduate Program in Dentistry, Federal University of Pelotas, Rua Gonçalves Chaves 457, Centro, 96015-560 Pelotas, RS, Brazil
2Department of Operative Dentistry School of Dentistry, Federal University of Pelotas, Rua Gonçalves Chaves 457, Centro, 96015-560 Pelotas, RS, Brazil
3Department of Operative Dentistry School of Dentistry, University North of Paraná (UNOPAR), Rua Marselha, Jardim Piza, 86041-140 Londrina, PR, Brazil
4Technology Development Center, Federal University of Pelotas, Rua Felix da Cunha 809, Centro, 96010-00 Pelotas, RS, Brazil

Received 16 March 2013; Accepted 10 May 2013

Academic Editor: Kacey Gribbin Marra

Copyright © 2013 Bianca Palma Santana et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. F. Nedel, D. D. A. André, I. O. de Oliveira et al., “Stem cells: therapeutic potential in dentistry,” The journal of contemporary dental practice, vol. 10, no. 4, pp. 90–96, 2009. View at Scopus
  2. F. Nedel, F. N. Soki, M. C. M. Conde et al., “Comparative analysis of two colorimetric assays in dental pulp cell density,” International Endodontic Journal, vol. 44, no. 1, pp. 59–64, 2011. View at Publisher · View at Google Scholar · View at Scopus
  3. B. P. Santana, G. F. D. R. Paganotto, F. Nedel, et al., “Nano-/microfiber scaffold for tissue engineering: physical and biological properties,” Journal of Biomedical Materials Research Part A, vol. 100, no. 11, pp. 3051–3058, 2012.
  4. R. Langer and J. P. Vacanti, “Tissue engineering,” Science, vol. 260, no. 5110, pp. 920–926, 1993. View at Scopus
  5. F. F. Demarco, M. C. M. Conde, B. N. Cavalcanti, L. Casagrande, V. T. Sakai, and J. E. Nör, “Dental pulp tissue engineering,” Brazilian Dental Journal, vol. 22, no. 1, pp. 3–14, 2011. View at Scopus
  6. F. P. Hartwig, F. Nedel, T. V. Collares, S. B. Tarquinio, J. E. Nör, and F. F. Demarco, “Telomeres and tissue engineering: the potential roles of TERT in VEGF-mediated angiogenesis,” Stem Cell Reviews and Reports, vol. 8, no. 4, pp. 1275–1281, 2012.
  7. J. Zhu, “Bioactive modification of poly(ethylene glycol) hydrogels for tissue engineering,” Biomaterials, vol. 31, no. 17, pp. 4639–4656, 2010. View at Publisher · View at Google Scholar · View at Scopus
  8. F. F. Demarco, L. Casagrande, Z. Zhang et al., “Effects of morphogen and scaffold porogen on the differentiation of dental pulp stem cells,” Journal of Endodontics, vol. 36, no. 11, pp. 1805–1811, 2010. View at Publisher · View at Google Scholar · View at Scopus
  9. K. M. Woo, V. J. Chen, and P. X. Ma, “Nano-fibrous scaffolding architecture selectively enhances protein adsorption contributing to cell attachment,” Journal of Biomedical Materials Research A, vol. 67, no. 2, pp. 531–537, 2003. View at Scopus
  10. K. M. Woo, J. Jun, V. J. Chen et al., “Nano-fibrous scaffolding promotes osteoblast differentiation and biomineralization,” Biomaterials, vol. 28, no. 2, pp. 335–343, 2007. View at Publisher · View at Google Scholar · View at Scopus
  11. B.-H. Choi, Y. S. Choi, D. G. Kang, B. J. Kim, Y. H. Song, and H. J. Cha, “Cell behavior on extracellular matrix mimic materials based on mussel adhesive protein fused with functional peptides,” Biomaterials, vol. 31, no. 34, pp. 8980–8988, 2010. View at Publisher · View at Google Scholar · View at Scopus
  12. L. A. Smith and P. X. Ma, “Nano-fibrous scaffolds for tissue engineering,” Colloids and Surfaces B, vol. 39, no. 3, pp. 125–131, 2004. View at Publisher · View at Google Scholar · View at Scopus
  13. K. Tuzlakoglu, N. Bolgen, A. J. Salgado, M. E. Gomes, E. Piskin, and R. L. Reis, “Nano- and micro-fiber combined scaffolds: a new architecture for bone tissue engineering,” Journal of Materials Science, vol. 16, no. 12, pp. 1099–1104, 2005. View at Publisher · View at Google Scholar · View at Scopus
  14. C.-Y. Yu, X.-C. Zhang, F.-Z. Zhou, X.-Z. Zhang, S.-X. Cheng, and R.-X. Zhuo, “Sustained release of antineoplastic drugs from chitosan-reinforced alginate microparticle drug delivery systems,” International Journal of Pharmaceutics, vol. 357, no. 1-2, pp. 15–21, 2008. View at Publisher · View at Google Scholar · View at Scopus
  15. H. H. Tønnesen and J. Karlsen, “Alginate in drug delivery systems,” Drug Development and Industrial Pharmacy, vol. 28, no. 6, pp. 621–630, 2002. View at Publisher · View at Google Scholar · View at Scopus
  16. C. Juliano, M. Cossu, P. Pigozzi, G. Rassu, and P. Giunchedi, “Preparation, in vitro characterization and preliminary in vivo evaluation of buccal polymeric films containing chlorhexidine,” AAPS PharmSciTech, vol. 9, no. 4, pp. 1153–1158, 2008. View at Publisher · View at Google Scholar · View at Scopus
  17. G. D. Nicodemus and S. J. Bryant, “Cell encapsulation in biodegradable hydrogels for tissue engineering applications,” Tissue Engineering B, vol. 14, no. 2, pp. 149–165, 2008. View at Publisher · View at Google Scholar · View at Scopus
  18. L. N. Novikova, A. Mosahebi, M. Wiberg, G. Terenghi, J. O. Kellerth, and L. N. Novikov, “Alginate hydrogel and matrigel as potential cell carriers for neurotransplantation,” Journal of Biomedical Materials Research A, vol. 77, no. 2, pp. 242–252, 2006. View at Publisher · View at Google Scholar · View at Scopus
  19. J. P. Frampton, M. R. Hynd, M. L. Shuler, and W. Shain, “Fabrication and optimization of alginate hydrogel constructs for use in 3D neural cell culture,” Biomedical Materials, vol. 6, no. 1, Article ID 015002, 2011. View at Publisher · View at Google Scholar · View at Scopus
  20. W. R. Gombotz and S. F. Wee, “Protein release from alginate matrices,” Advanced Drug Delivery Reviews, vol. 31, no. 3, pp. 267–285, 1998. View at Publisher · View at Google Scholar · View at Scopus
  21. H. Park, S. W. Kang, B. Kim, D. J. Mooney, and K. Y. Lee, “Shear-reversibly crosslinked alginate hydrogels for tissue engineering,” Macromolecular Bioscience, vol. 9, no. 9, pp. 895–901, 2009. View at Publisher · View at Google Scholar · View at Scopus
  22. A. C. Jen, M. C. Wake, and A. G. Mikos, “Review: hydrogels for cell immobilization,” Biotechnology and Bioengineering, vol. 50, no. 4, pp. 357–364, 1996.
  23. T. Sone, E. Nagamori, T. Ikeuchi et al., “A novel gene delivery system in plants with calcium alginate micro-beads,” Journal of Bioscience and Bioengineering, vol. 94, no. 1, pp. 87–91, 2002. View at Publisher · View at Google Scholar · View at Scopus
  24. K. Dobie, G. Smith, A. J. Sloan, and A. J. Smith, “Effects of alginate hydrogels and TGF-β1 on human dental pulp repair in vitro,” Connective Tissue Research, vol. 43, no. 2-3, pp. 387–390, 2002. View at Scopus
  25. J. W. Lee, Y. J. Park, S. J. Lee, S. K. Lee, and K. Y. Lee, “The effect of spacer arm length of an adhesion ligand coupled to an alginate gel on the control of fibroblast phenotype,” Biomaterials, vol. 31, no. 21, pp. 5545–5551, 2010. View at Publisher · View at Google Scholar · View at Scopus
  26. S. Henn, F. Nedel, R. V. de Carvalho et al., “Characterization of an antimicrobial dental resin adhesive containing zinc methacrylate,” Journal of Materials Science, vol. 22, no. 8, pp. 1797–1802, 2011. View at Publisher · View at Google Scholar · View at Scopus
  27. F. Nedel, K. Begnini, P. H. Carvalho, R. G. Lund, F. T. Beira, and F. A. del Pino, “Antiproliferative activity of flower hexane extract obtained from mentha spicata associated with mentha rotundifolia against the MCF7, KB, and NIH/3T3 Cell Lines,” Journal of Medicinal Food, vol. 15, no. 11, pp. 955–958, 2012.
  28. S. I. Jeong, M. D. Krebs, C. A. Bonino, S. A. Khan, and E. Alsberg, “Electrospun alginate nanofibers with controlled cell adhesion for tissue engineering,” Macromolecular Bioscience, vol. 10, no. 8, pp. 934–943, 2010. View at Publisher · View at Google Scholar · View at Scopus
  29. J. L. Drury and D. J. Mooney, “Hydrogels for tissue engineering: scaffold design variables and applications,” Biomaterials, vol. 24, no. 24, pp. 4337–4351, 2003. View at Publisher · View at Google Scholar · View at Scopus
  30. K. Cai, A. Rechtenbach, J. Hao, J. Bossert, and K. D. Jandt, “Polysaccharide-protein surface modification of titanium via a layer-by-layer technique: characterization and cell behaviour aspects,” Biomaterials, vol. 26, no. 30, pp. 5960–5971, 2005. View at Publisher · View at Google Scholar · View at Scopus
  31. J. Choi, T. Konno, R. Matsuno, M. Takai, and K. Ishihara, “Surface immobilization of biocompatible phospholipid polymer multilayered hydrogel on titanium alloy,” Colloids and Surfaces B, vol. 67, no. 2, pp. 216–223, 2008. View at Publisher · View at Google Scholar · View at Scopus
  32. S. Oh, K. S. Brammer, Y. S. J. Li et al., “Stem cell fate dictated solely by altered nanotube dimension,” Proceedings of the National Academy of Sciences of the United States of America, vol. 106, no. 7, pp. 2130–2135, 2009. View at Publisher · View at Google Scholar · View at Scopus
  33. B. E. Rapuano, J. J. E. Lee, and D. E. Macdonald, “Titanium alloy surface oxide modulates the conformation of adsorbed fibronectin to enhance its binding to α5β1 integrins in osteoblasts,” European Journal of Oral Sciences, vol. 120, no. 3, pp. 185–194, 2012. View at Publisher · View at Google Scholar · View at Scopus
  34. J. F. Blanco, F. M. Sánchez-Guijo, S. Carrancio, S. Muntion, J. García-Briñon, and M. del Cañizo, “Titanium and tantalum as mesenchymal stem cell scaffolds for spinal fusion: an in vitro comparative study,” European Spine Journal, vol. 20, no. 3, Supplemnt, pp. 353–360, 2011. View at Publisher · View at Google Scholar · View at Scopus
  35. M. Gómez-Florit, M. Rubert, J. M. Ramis, et al., “TiO2 Scaffolds Sustain Differentiation of MC3T3-E1 Cells,” Journal of Biomaterials and Tissue Engineering, vol. 2, no. 4, pp. 336–344, 2012.
  36. M. Schindler, I. Ahmed, J. Kamal et al., “A synthetic nanofibrillar matrix promotes in vivo-like organization and morphogenesis for cells in culture,” Biomaterials, vol. 26, no. 28, pp. 5624–5631, 2005. View at Publisher · View at Google Scholar · View at Scopus
  37. K. Park, Y. M. Ju, J. S. Son, K. Ahn, and D. K. Han, “Surface modification of biodegradable electrospun nanofiber scaffolds and their interaction with fibroblasts,” Journal of Biomaterials Science, vol. 18, no. 4, pp. 369–382, 2007. View at Publisher · View at Google Scholar · View at Scopus
  38. C. Y. Xu, R. Inai, M. Kotaki, and S. Ramakrishna, “Aligned biodegradable nanofibrous structure: a potential scaffold for blood vessel engineering,” Biomaterials, vol. 25, no. 5, pp. 877–886, 2004. View at Publisher · View at Google Scholar · View at Scopus
  39. F. Yang, R. Murugan, S. Wang, and S. Ramakrishna, “Electrospinning of nano/micro scale poly(l-lactic acid) aligned fibers and their potential in neural tissue engineering,” Biomaterials, vol. 26, no. 15, pp. 2603–2610, 2005. View at Publisher · View at Google Scholar · View at Scopus