Dual Inhibiting Senescence and Epithelial-to-Mesenchymal Transition by Erythropoietin Preserve Tubular Epithelial Cell Regeneration and Ameliorate Renal Fibrosis in Unilateral Ureteral Obstruction
Figure 3
Representative photographs of kidney sections stained with TGF-β, BMP-7, Smad2/3, and in UUO model. In sham kidneys, no or little TGF-β labelling was seen. Advanced increased TGF-β labelling was seen in the interstitium area in the obstructed kidneys compared with the sham at days 3, 7, and 14. In contrast, decrease of TGF-β staining was observed in UUO mice with rhEPO treatment. In contrast, BMP-7 was demonstrated in the cytoplasm of TEC in sham kidneys, whereas the labeling of BMP-7 was decreased in cytoplasm of TEC particularly in dilated and atrophic tubules of the placebo treated UUO kidneys since day 3 after UUO and progressive loss until day 14. rhEPO treatment in mice with UUO demonstrated the significantly preserved cytoplasm staining intensity of BMP-7 in the obstructed kidneys. Moreover, no Smad2/3 and staining was seen in TEC in sham kidneys. Smad2/3 and are detected at the nucleus of TEC with weak cytoplasm staining particularly in dilated and atrophic tubules of the UUO kidneys since days 3, 7, and 14. In contrast, rhEPO treatment in mice with UUO demonstrated the significantly attenuated nucleus and cytoplasm staining intensity of Smad2/3 and in the obstructed kidneys. Original magnifications ×400.
