308515.fig.003a
(a)
308515.fig.003b
(b)
308515.fig.003c
(c)
308515.fig.003d
(d)
308515.fig.003e
(e)
308515.fig.003f
(f)
Figure 3: Effect of crude extract of endophytic Pestalotiopsis sp. on the viability of protoscoleces of Echinococcus granulosus. (a) Control unstained invaginated protoscoleces of hydatid cyst by wet mount drop (10×). (b) Extract-treated (20 mg/mL) hydatid cysts loose their viability and take stain eosin red. ((a)-(b)) represents corresponding mortality observed in control and treated protoscoleces. (c) The lipid granule formation is characteristic feature of the starvation of the protoscoleces. ((d)–(f)) Dead stained protoscoleces after treatment with extract of endophytic Pestalotiopsis sp. (f) Extracts showed distinct morphological distortions and degenerative effects and remarkably distinct digitiform tegumental extension after 30 min treatment.