308515.fig.004
Figure 4: Comparative evaluation of the tegumental and morphological damage in viable and dead protoscoleces after treatment with extract of Pestalotiopsis sp. The inset figure represents the treatment with eosin red dye. (a) Control live unstained protoscoleces showing characteristic smooth-walled tegument. (b) Pestalotiopsis sp. extract-treated (20 mg/mL; 10 min) protoscoleces showed marked damage in the tegumental layer (notice the inset area). (c) The cavity slide showing the stained protoscoleces preparation for the microscopic examination.