Streptomyces lunalinharesii Strain 235 Shows the Potential to Inhibit Bacteria Involved in Biocorrosion Processes

Table 2

Properties of the antimicrobial substances produced by Streptomyces  lunalinharesii 235.

Responses to	Sensitive^b
Enzymes^a
Pronase E	−
Proteinase K	+
Trypsin	+
Solvents (10% and 50%)
Methanol	−
Ethanol	−
Acetone	−
Chloroform	−
Chemicals
Urea 6 M	−
NaOH (0.2 M and 1 M)	−
HCl 0.2 M	−
Heat treatment
40°C for 20, 45 and 60 min	−
60°C for 20, 45 and 60 min	−
80°C for 20, 45 and 60 min	−
100°C for 20, 45 and 60 min	−
Autoclavation (121°C for 20 min)	+
pH^c
3.0–6.0	−
7.0–9.0	−

^aAll enzymes were used at final concentration of 1 mg/mL, and tested with 300-fold concentrated supernatant containing the AMS.
^b(−) inhibition zones similar to those observed in control without treatment, (+) no inhibition zone observed.
^cpH of supernatants was adjusted from 3.0 to 9.0 varying 1.0 unit before testing their activity.