Table 2: Properties of the antimicrobial substances produced by Streptomyces  lunalinharesii 235.

Responses to Sensitiveb

Enzymesa
 Pronase E
 Proteinase K+
 Trypsin+
Solvents (10% and 50%)
 Methanol
 Ethanol
 Acetone
 Chloroform
Chemicals
 Urea 6 M
 NaOH (0.2 M and 1 M)
 HCl 0.2 M
Heat treatment
 40°C for 20, 45 and 60 min
 60°C for 20, 45 and 60 min
 80°C for 20, 45 and 60 min
 100°C for 20, 45 and 60 min
 Autoclavation (121°C for 20 min)+
pHc
 3.0–6.0
 7.0–9.0

aAll enzymes were used at final concentration of 1 mg/mL, and tested with 300-fold concentrated supernatant containing the AMS.
b(−) inhibition zones similar to those observed in control without treatment, (+) no inhibition zone observed.
cpH of supernatants was adjusted from 3.0 to 9.0 varying 1.0 unit before testing their activity.