Assessing the Detection Capacity of Microarrays as Bio/Nanosensing Platforms

Table 1

Limit of detection and quantification (LOD and LOQ, resp.), which were calculated through detection limit theory with prediction and tolerance interval.

	Weighted prediction intervals^a		Weighted tolerance intervals^b
	LOD	LOQ	LOD	LOQ
	Number of fluorophores/𝜇m^2
Cy3	13,366	36,606	21,046	42,666
Cy5	16,487	39,680	26,047	46,440

a99% confidence (i.e., 𝛼=𝛽=0.01).
^b99% confidence and 99% coverage (i.e., 𝛼=𝛽=0.01 and 𝑃=0.99).