Preparative Purification of Recombinant Proteins: Current Status and Future Trends

Table 2

Selected commercially available mix-mode media.

Media	Supplier	Type	Ligand	pH stability
CHT ceramic hydroxyapatite	Bio-Rad laboratories	Ion exchange, metal chelation	[Ca5[PO4]3OH]2	Operating pH: 5.5–14; Can be cleaned with 1-2 M NaOH
CHT Fluorapatite	Bio-Rad laboratories	Ion exchange, metal chelation	[Ca10[PO4]6F]2	Operating pH: 5–14; Can be cleaned with 1-2 M NaOH
MEP	Pall life sciences	Hydrophobic binding near neutral pH, elution by pH reduction	4-Mercapto ethyl pyridine	Working pH: 2–12; Cleaning pH: 2–14
HEA	Pall life sciences	Hydrophobic binding near neutral pH, elution by pH reduction	Hexylamino	Working pH: 2–12; Cleaning pH: 1–14
PPA	Pall life sciences	Hydrophobic binding near neutral pH, elution by pH reduction	Phenylpropylamino	Working pH: 2–12; Cleaning pH: 1–14
MBI	Pall life sciences	Hydrophobic binding at acidic pH, elution by raising the pH	2-Mercapto-5-benzimidazole sulfonic acid	—
Capto MMC	GE Healthcare	Multimodal cation exchange	2-Benzamido-4-mercaptobutanoic acid	Long term: 2–12; Short term: 2–14
Capto adhere	GE Healthcare	Multimodal strong anion exchange	N-benzyl-N-methyl ethanolamine	Long term: 3–12; Short term: 2–14