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BioMed Research International
Volume 2013 (2013), Article ID 314654, 11 pages
http://dx.doi.org/10.1155/2013/314654
Research Article

Staphylococcus aureus Clinical Isolates: Antibiotic Susceptibility, Molecular Characteristics, and Ability to Form Biofilm

1Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand
2Department of Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand
3Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand
4Natural Products Research Center and Department of Microbiology, Faculty of Science, Prince of Songkla University, Songkhla 90112, Thailand
5Department of Applied Veterinary Medicine and Public Health, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080 8555, Japan

Received 30 May 2013; Accepted 24 July 2013

Academic Editor: Sou-ichi Makino

Copyright © 2013 N. Indrawattana et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. R. E. Williams, “Healthy carriage of Staphylococcus aureus: its prevalence and importance,” Bacteriological reviews, vol. 27, pp. 56–71, 1963. View at Scopus
  2. D. L. Stulberg, M. A. Penrod, and R. A. Blatny, “Common bacterial skin infections,” The American Family Physician, vol. 66, no. 1, pp. 119–124, 2002. View at Scopus
  3. I. V. Pinchuk, E. J. Beswick, and V. E. Reyes, “Staphylococcal enterotoxins,” Toxins, vol. 2, no. 8, pp. 2177–2197, 2010. View at Publisher · View at Google Scholar · View at Scopus
  4. S. D. Haessler and R. B. Brown, “Pneumonia caused by Staphylococcus aureus,” Current Respiratory Medicine Reviews, vol. 5, no. 1, pp. 62–67, 2009. View at Publisher · View at Google Scholar · View at Scopus
  5. M. Kowalik, J. Ellert-Zygadłowska, T. Smiatacz et al., “Staphylococcus aureus sepsis: still life threatening disease,” Polski Merkuriusz Lekarski, vol. 11, no. 64, pp. 352–356, 2001. View at Scopus
  6. T. Proft and J. D. Fraser, “Bacterial superantigens,” Clinical and Experimental Immunology, vol. 133, no. 3, pp. 299–306, 2003. View at Publisher · View at Google Scholar · View at Scopus
  7. M. M. Dinges, P. M. Orwin, and P. M. Schlievert, “Exotoxins of Staphylococcus aureus,” Clinical Microbiology Reviews, vol. 13, no. 1, pp. 16–34, 2000. View at Scopus
  8. N. Balaban and A. Rasooly, “Staphylococcal enterotoxins,” International Journal of Food Microbiology, vol. 61, no. 1, pp. 1–10, 2000. View at Publisher · View at Google Scholar · View at Scopus
  9. M. Llewelyn and J. Cohen, “Superantigens: microbial agents that corrupt immunity,” The Lancet Infectious Diseases, vol. 2, no. 3, pp. 156–162, 2002. View at Publisher · View at Google Scholar · View at Scopus
  10. G. Lina, G. A. Bohach, S. P. Nair, K. Hiramatsu, E. Jouvin-Marche, and R. Mariuzza, “Standard nomenclature for the superantigens expressed by Staphylococcus,” Journal of Infectious Diseases, vol. 189, no. 12, pp. 2334–2336, 2004. View at Publisher · View at Google Scholar · View at Scopus
  11. M. Á. Argudín, M. C. Mendoza, and M. R. Rodicio, “Food poisoning and Staphylococcus aureus enterotoxins,” Toxins, vol. 2, no. 7, pp. 1751–1773, 2010. View at Publisher · View at Google Scholar · View at Scopus
  12. M. S. Bergdoll, B. A. Crass, R. F. Reiser, R. N. Robbins, and J. P. Davis, “A new staphylococcal enterotoxin, enterotoxin F, associated with toxic-shock-syndrome Staphylococcus aureus isolates,” The Lancet, vol. 1, no. 8228, pp. 1017–1021, 1981. View at Scopus
  13. S. Holtfreter, D. Grumann, M. Schmudde et al., “Clonal distribution of superantigen genes in clinical Staphylococcus aureus isolates,” Journal of Clinical Microbiology, vol. 45, no. 8, pp. 2669–2680, 2007. View at Publisher · View at Google Scholar · View at Scopus
  14. R. P. Novick, H. F. Ross, S. J. Projan, J. Kornblum, B. Kreiswirth, and S. Moghazeh, “Synthesis of staphylococcal virulence factors is controlled by a regulatory RNA molecule,” EMBO Journal, vol. 12, no. 10, pp. 3967–3975, 1993. View at Scopus
  15. N. Balaban and R. P. Novick, “Translation of RNAIII, the Staphylococcus aureus, agr regulatory RNA molecule, can be activated by a 3′-end deletion,” FEMS Microbiology Letters, vol. 133, no. 1-2, pp. 155–161, 1995. View at Publisher · View at Google Scholar · View at Scopus
  16. P. Gilot, G. Lina, T. Cochard, and B. Poutrel, “Analysis of the genetic variability of genes encoding the RNA III-activating components Agr and TRAP in a population of Staphylococcus aureus strains isolated from cows with mastitis,” Journal of Clinical Microbiology, vol. 40, no. 11, pp. 4060–4067, 2002. View at Publisher · View at Google Scholar · View at Scopus
  17. F. D. Lowy, “Medical progress: Staphylococcus aureus infections,” The New England Journal of Medicine, vol. 339, no. 8, pp. 520–532, 1998. View at Publisher · View at Google Scholar · View at Scopus
  18. F. D. Lowy, “Antimicrobial resistance: the example of Staphylococcus aureus,” Journal of Clinical Investigation, vol. 111, no. 9, pp. 1265–1273, 2003. View at Publisher · View at Google Scholar · View at Scopus
  19. Y. Katayama, T. Ito, and K. Hiramatsu, “A new class of genetic element, staphylococcus cassette chromosome mec, encodes methicillin resistance in Staphylococcus aureus,” Antimicrobial Agents and Chemotherapy, vol. 44, no. 6, pp. 1549–1555, 2000. View at Publisher · View at Google Scholar · View at Scopus
  20. The committee on Using Computer in Clinical Microbiology, “Bacterial antimicrobial susceptibility pattern 1998,” Journal of Infectious Diseases and Antimicrobial Agents, vol. 8, pp. 25–39, 1991.
  21. “Drug resistance surveillance 1998, pilot phase report,” National Antimicrobial Resistance Surveillance Center, Ministry of Public Health, Thailand, 1999.
  22. S. Wongwanich, P. Tishyadhigama, S. Paisomboon, T. Ohta, and H. Hayashi, “Epidemiological analysis of methicillin resistant Staphylococcus aureus in Thailand,” Southeast Asian Journal of Tropical Medicine and Public Health, vol. 31, no. 1, pp. 72–76, 2000. View at Scopus
  23. S. Trakulsomboon, S. Danchaivijitr, Y. Rongrungruang et al., “First report of methicillin-resistant Staphylococcus aureus with reduced susceptibility to vancomycin in Thailand,” Journal of Clinical Microbiology, vol. 39, no. 2, pp. 591–595, 2001. View at Publisher · View at Google Scholar · View at Scopus
  24. CLSI, “Performance standards for antimicrobial susceptibility testing, 20th informational supplement,” CLSI Document M100-S20, CLSI, Wayne, Pa, USA, 2010.
  25. S. Jarraud, G. J. Lyon, A. M. S. Figueiredo et al., “Exfoliatin-producing strains define a fourth agr specificity group in Staphylococcus aureus,” Journal of Bacteriology, vol. 182, no. 22, pp. 6517–6522, 2000. View at Publisher · View at Google Scholar · View at Scopus
  26. D. Wu, X. Li, Y. Yang et al., “Superantigen gene profiles and presence of exfoliative toxin genes in community-acquired meticillin-resistant Staphylococcus aureus isolated from Chinese children,” Journal of Medical Microbiology, vol. 60, no. 1, pp. 35–45, 2011. View at Publisher · View at Google Scholar · View at Scopus
  27. S. J. Peacock, G. D. I. de Silva, A. Justice et al., “Comparison of multilocus sequence typing and pulsed-field gel electrophoresis as tools for typing Staphylococcus aureus isolates in a microepidemiological setting,” Journal of Clinical Microbiology, vol. 40, no. 10, pp. 3764–3770, 2002. View at Publisher · View at Google Scholar · View at Scopus
  28. L. Wang, F. Yu, L. Yang et al., “Prevalence of virulence genes and biofilm formation among Staphylococcus aureus clinical isolates associated with lower respiratory infection,” African Journal of Microbiology Research, vol. 4, no. 23, pp. 2566–2569, 2010. View at Scopus
  29. M. P. Jevons, “Celbenin-resistant staphylococci,” British Medical Journal, vol. 1, pp. 24–25, 1961.
  30. J. B. Kaper and J. Hacker, Pathogenicity Islands and Other Mobile Virulence Elements, The American Society for Microbiology Press, Washington, DC, USA, 1999.
  31. S. Chang, D. M. Sievert, J. C. Hageman et al., “Infection with vancomycin-resistant Staphylococcus aureus containing the vanA resistance gene,” The New England Journal of Medicine, vol. 348, no. 14, pp. 1342–1347, 2003. View at Publisher · View at Google Scholar · View at Scopus
  32. S. Christopher, R. M. Verghis, B. Antonisamy et al., “Transmission dynamics of methicillin-resistant Staphylococcus aureus in a medical intensive care unit in India,” PLoS ONE, vol. 6, no. 7, Article ID e20604, 2011. View at Publisher · View at Google Scholar · View at Scopus
  33. J. Sila, P. Sauer, and M. Kolar, “Comparison of the prevalence of genes coding for enterotoxins, exfoliatins, Panton-Valentine leukocidin and TSST-1 between methicillin-resistant and methicillin-susceptible isolates of Staphylococcus aureus at the University Hospital in Olomouc,” Biomedical Papers, vol. 153, no. 3, pp. 215–218, 2009. View at Scopus
  34. K. T. Lim, Y. A. Hanifah, M. Y. Mohd Yusof, and K. L. Thong, “Investigation of toxin genes among methicillin-resistant Staphylococcus aureus strains isolated from a tertiary hospital in Malaysia,” Tropical Biomedicine, vol. 29, pp. 212–219, 2012.
  35. J. N. Jiménez, A. M. Ocampo, J. M. Vanegas et al., “Characterisation of virulence genes in methicillin susceptible and resistant Staphylococcus aureus isolates from a paediatric population in a university hospital of Medellín, Colombia,” Memórias do Instituto Oswaldo Cruz, vol. 106, pp. 980–985, 2011.
  36. S. Jarraud, M. A. Peyrat, A. Lim et al., “egc, a highly prevalent operon of enterotoxin gene, forms a putative nursery of superantigens in Staphylococcus aureus,” Journal of Immunology, vol. 166, no. 1, pp. 669–677, 2001. View at Scopus
  37. M. Mempel, G. Lina, M. Hojka et al., “High prevalence of superantigens associated with the egc locus in Staphylococcus aureus isolates from patients with atopic eczema,” European Journal of Clinical Microbiology and Infectious Diseases, vol. 22, no. 5, pp. 306–309, 2003. View at Scopus
  38. K. Becker, A. W. Friedrich, G. Lubritz, M. Weilert, G. Peters, and C. von Eiff, “Prevalence of genes encoding pyrogenic toxin superantigens and exfoliative toxins among strains of Staphylococcus aureus isolated from blood and nasal specimens,” Journal of Clinical Microbiology, vol. 41, no. 4, pp. 1434–1439, 2003. View at Publisher · View at Google Scholar · View at Scopus
  39. K. Omoe, D.-L. Hu, H. Takahashi-Omoe, A. Nakane, and K. Shinagawa, “Comprehensive analysis of classical and newly described staphylococcal superantigenic toxin genes in Staphylococcus aureus isolates,” FEMS Microbiology Letters, vol. 246, no. 2, pp. 191–198, 2005. View at Publisher · View at Google Scholar · View at Scopus
  40. A. Bendahou, M. Abid, N. Bouteldoun, D. Catelejine, and M. Lebbadi, “Enterotoxigenic coagulase positive Staphylococcus in milk and milk products, Lben and Jben, in northern Morocco,” Journal of Infection in Developing Countries, vol. 3, no. 3, pp. 169–176, 2009. View at Scopus
  41. C. A. Genigeorgis, “Present state of knowledge on staphylococcal intoxication,” International Journal of Food Microbiology, vol. 9, no. 4, pp. 327–360, 1989. View at Scopus
  42. L. Chapaval, D. H. Moon, J. E. Gomes, F. R. Duarte, and S. M. Tsai, “Use of PCR to detect classical enterotoxin genes (ent) and toxic shock syndrome toxin-1 gene (tst) in Staphylocooccus aureus isolated from crude milk and determination of toxin productivities of S. aureus isolates harboring these gene,” Arquivos Do Instituto Biológico, vol. 73, pp. 165–169, 2006.
  43. N. K. Sharma, C. E. D. Rees, and C. E. R. Dodd, “Development of a single-reaction multiplex PCR toxin typing assay for Staphylococcus aureus strains,” Applied and Environmental Microbiology, vol. 66, no. 4, pp. 1347–1353, 2000. View at Publisher · View at Google Scholar · View at Scopus
  44. Y. Xie, Y. He, A. Gehring et al., “Genotypes and toxin gene profiles of Staphylococcus aureus clinical isolates from China,” PLoS ONE, vol. 6, no. 12, Article ID e28276, 2011. View at Publisher · View at Google Scholar · View at Scopus
  45. Y. Shimamura and M. Murata, “Pulsed-field gel electrophoretic analysis and some characteristics of Staphylococcus aureus isolated from retail foods and human hands,” Bioscience, Biotechnology and Biochemistry, vol. 75, no. 6, pp. 1177–1180, 2011. View at Publisher · View at Google Scholar · View at Scopus
  46. G. Ji, R. Beavis, and R. P. Novick, “Bacterial interference caused by autoinducing peptide variants,” Science, vol. 276, no. 5321, pp. 2027–2030, 1997. View at Publisher · View at Google Scholar · View at Scopus
  47. W. van Leeuwen, W. van Nieuwenhuizen, C. Gijzen, H. Verbrugh, and A. van Belkum, “Population studies of methicillin-resistant and -sensitive Staphylococcus aureus strains reveal a lack of variability in the agrD gene, encoding a staphylococcal autoinducer peptide,” Journal of Bacteriology, vol. 182, no. 20, pp. 5721–5729, 2000. View at Publisher · View at Google Scholar · View at Scopus
  48. S. Jarraud, C. Mougel, J. Thioulouse et al., “Relationships between Staphylococcus aureus genetic background, virulence factors, agr groups (alleles), and human disease,” Infection and Immunity, vol. 70, no. 2, pp. 631–641, 2002. View at Publisher · View at Google Scholar · View at Scopus
  49. G. F. M. Gad, M. A. El-Feky, M. S. El-Rehewy, M. A. Hassan, H. Abolella, and R. M. A. El-Baky, “Detection of icaA, icaD genes and biofilm production by Staphylococcus aureus and Staphylococcus epidermidis isolated from urinary tract catheterized patients,” Journal of Infection in Developing Countries, vol. 3, no. 5, pp. 342–351, 2009. View at Scopus