Effect of Supracervical Apposition and Spontaneous Labour on Apoptosis and Matrix Metalloproteinases in Human Fetal Membranes
Table 2
Intensity and extent of staining of apoptotic markers from non-labouring and post-labour SC and distal fetal membranes.
Antigen
Tissue
Intensity of staining
Extent of staining
No-labour
Post-labour
No-labour
Post-labour
DS
SCS
SOR
DS
SCS
SOR
Intrinsic pathway
Bax
Amnion
1.8 ± 0.2
1.7 ± 0.2
1.2 ± 0.2
3.2 ± 0.0
3.8 ± 0.2
1.8 ± 0.2*
Chorion
2.0 ± 0.0
2.0 ± 0.0
2.0 ± 0.4
3.2 ± 0.4*
4.2 ± 0.3
2.5 ± 0.2*
Smac
Amnion
0.0 ± 0.0*
0.8 ± 0.2
0.8 ± 0.8
0.0 ± 0.0
0.7 ± 0.3
0.8 ± 0.9
Chorion
1.0 ± 0.0*
1.7 ± 0.2
1.5 ± 0.2
1.8 ± 0.4*
3.5 ± 0.2
2.3 ± 0.6
Extrinsic pathway
Fas
Amnion
1.8 ± 0.2
1.8 ± 0.2
1.8 ± 0.2
4.3 ± 0.2
3.8 ± 0.3
4.0 ± 0.3
Chorion
3.3 ± 0.3
2.0 ± 0.5
2.0 ± 0.2
3.5 ± 0.3*
4.3 ± 0.2
4.0 ± 0.4
FasL
Amnion
2.0 ± 0.0
2.0 ± 0.3
2.0 ± 0.0
1.7 ± 0.2
1.8 ± 0.5
2.5 ± 0.4
Chorion
2.0 ± 0.0
2.5 ± 0.2
2.0 ± 0.2
3.0 ± 0.0*
4.0 ± 0.3
3.0 ± 0.3*
Common terminal pathway
Caspase-3
Amnion
0.0 ± 0.0*
0.7 ± 0.2
0.5 ± 0.2
0.0 ± 0.0
0.5 ± 0.2
0.7 ± 0.3
Chorion
1.0 ± 0.0*
1.7 ± 0.2
1.0 ± 0.0*
1.8 ± 0.3*
3.5 ± 0.2
2.7 ± 0.5
PARP
Amnion
0.0 ± 0.0
0.0 ± 0.0
0.0 ± 0.0
0.0 ± 0.0
0.0 ± 0.0
0.0 ± 0.0
Chorion
2.0 ± 0.0
1.8 ± 0.3
1.2 ± 0.2
3.0 ± 0.3*
4.0 ± 0.3
0.8 ± 0.3*
Antiapoptotic
Bcl-2
Amnion
0.0 ± 0.0
0.0 ± 0.0
0.0 ± 0.0
0.0 ± 0.0
0.0 ± 0.0
0.0 ± 0.0
Chorion
0.0 ± 0.0
0.17 ± 0.2
0.0 ± 0.0
0.0 ± 0.0
0.0 ± 0.0
0.0 ± 0.0
All data is expressed as mean ± SEM ( patients per group). versus SCS (no-labour SCS versus no-labour DS analysed by paired sample comparison; no-labour SCS versus post-labour SOR analysed by Student’s -test).
