Table 2: Sequences of the adapters and primers.

Name of primersSequences of primers (5′-3′)

PstI adapterSense strand: CTC GGA AGC CTC AGT CCC AGA CTG CGT ACA TGC A-OH
Antisense strand: phos-TGT ACG CAG TCT GGG ACT GAG GCT TCC GAG A-OH

MseI adapter Sense strand: GAG CAA GGC TCT CAC AAG GAC GAC CGA CGA G-OH
Antisense strand: phos-TAC TCG TCG GTC GTC CTT GTG AGA GCC TTG CT-OH.

MseI suppressed amplification primerGAG CAA GGC TCT CAC A

PstI suppression amplification primerCTC GGA AGC CTC AGT C

MseI preamplification primerGAC GAC CGA CGA GTA AC

PstI preamplification primerAGA CTG CGT ACA TGC AGG A

PstI SAM primers Adapter 1: AGA CTG CGT ACA TGC AGG ACC
Adapter 2: AGA CTG CGT ACA TGC AGG ACG
Adapter 3: AGA CTG CGT ACA TGC AGG AGC
Adapter 4: AGA CTG CGT ACA TGC AGG A CT
Adapter 5: AGA CTG CGT ACA TGC AGG A TC
Adapter 6: AGA CTG CGT ACA TGC AGG A CA
Adapter 7: AGA CTG CGT ACA TGC AGG A AC
Adapter 8: AGA CTG CGT ACA TGC AGG A GT
Adapter 9: AGA CTG CGT ACA TGC AGG A TG
Adapter 10: AGA CTG CGT ACA TGC AGG A GA
Adapter 11: AGA CTG CGT ACA TGC AGG A AG
Adapter 12: AGA CTG CGT ACA TGC AGG A AT

5′-Anchored SSR primersPAC: a: KKR YRY YAC ACA CAC ACA C
  b: KKY RYR YCA CAC ACA CAC A
PCT: a: KKV RVR VCT CTC TCT CTC T
  b: KKR VRV RTC TCT CTC TCT C

K = G/T, R = G/A, Y = T/C, V = G/C/A, H = A/C/T for 5′-anchored SSR primer sequences.