Table 3: Genotype frequencies of single nucleotide polymorphisms (SNPs) rs4986790, rs4986791, rs11536858, rs1927911, and rs1927914 of TLR4 gene among Diabetic Foot Ulcer (DFU) and controls. The differences in frequencies between the DFU and control groups were analyzed for statistical significance at the 95% confidence interval using chi square test. Odds ratios (ORs) were calculated and reported within the 95% confidence limits. A two-tailed value of ≤0.05 was considered as statistically significant.

SNP and genotype Controls, number, (%) Cases, number, (%) OR 95% CI value

rs4986790 (TLR4_Asp299Gly) 119515123
AA 101 (77.7) 83 (66.4)
AG 29 (22.3) 41 (32.8) 1.7 1.0 to 3.0 0.05
GG 00 (00) 1 (0.8)
AG + GG29 (22.3) 42 (33.6) 1.75 1.01 to 3.02 0.04

rs4986791 (TLR4_Thr399Ile) 119515423
CC109 (83.8) 74 (59.2)
CT19 (14.6) 45 (36.0) 3.3 1.9 to 5.8 0.00003
TT2 (1.6) 6 (4.8) 4.1 1.0 to 17.2 0.05
CT + TT21 (16.2) 51 (40.8) 3.35 1.9 to 5.8 0.000003

rs11536858 (TLR4_1859) 120464147
AA 68 (52.3) 48 (38.4)
AG 59 (45.3) 68 (54.4) 1.6 1.0 to 2.7 0.05
GG 3 (2.4) 9 (7.2) 3.8 1.2 to 12.7 0.02
AG + GG 62 (47.7) 77 (61.6) 1.7 1.06 to 2.86 0.02

rs1927914 (TLR4_2437) 120464725
TT65 (50) 63 (50.4)
TC64 (49.2) 50 (40.0) 0.8 0.5 to 1.3 0.40
CC1 (0.8) 12 (9.6) 5.6 1.8 to 17.4 0.003
TC + CC65 (50) 62 (49.6) 0.98 0.6 to 1.6 0.94

rs1927911 (TLR4_7764) 120470054
CC63 (48.5) 40 (32.0)
CT52 (40) 66 (52.8) 2.0 1.2 to 3.3 0.011
TT15 (11.5) 19 (15.2) 2.0 0.9 to 4.3 0.08
CT + TT 67 (51.5) 85 (68.0) 2.0 1.2 to 3.2 0.007