[Retracted] Construction and Immunogenicity of DNA Vaccines Encoding Fusion Protein of Porcine IFN-𝝀1 and GP5 Gene of Porcine Reproductive and Respiratory Syndrome Virus

Figure 2

(a) Subcloning of the PoIFN-λ1 gene. Lane 1: PCR products of PoIFN-λ1; lane M: marker DL2000. (b) pMD¹⁸-T-PoIFN-λ1 digested with Nhe I and Kpn I. Lane 1: pMD¹⁸-T-PoIFN-λ1; lane M: Marker DL2000. (c) pcDNA3.1-PoIFN-λ1-SynORF5, pcDNA3.1-SynORF5, and the empty vector pcDNA3.1 digested with Nhe I/Kpn I, Kpn I/Xho I, and Nhe I/Xho I, respectively. Lane 1–3: pcDNA3.1-PoIFN-λ1-SynORF5, lane 4–6: pcDNA3.1-SynORF5, lane 7–9: pcDNA3.1, lane M1: marker DL (2000), and lane M2: Marker DL (5000).

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(b)

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