DHA Inhibits Protein Degradation More Efficiently than EPA by Regulating the PPARγ/NFκB Pathway in C2C12 Myotubes
Figure 3
Effects of n-3 PUFA on PPARγ gene expression. C2C12 myotubes were incubated with 600 μM EPA, 600 μM DHA, or 600 μM BSA for 24 hours. The PPARγ mRNA was determined using real-time PCR analysis and relative abundance of mRNA was calculated after normalization to β-actin. PPARγ = peroxisome proliferator-activated receptor γ, BSA = bovine serum albumin, DHA = docosahexaenoic acid, and EPA= eicosapentaenoic acid. Data are expressed as mean ± S.D. of 3 different experiments. ** versus control.