Regulatory Interactions between Androgens, Hoxb5, and TGFβ Signaling in Murine Lung Development
Figure 6
In vivo DHT treatment changed the spatial distribution of SMAD2P and SMAD7 proteins in E18 fetal mouse lungs. (a, a′, b, b′) SMAD2P; (c, d) SMAD7. Square inset in (a, b) represents areas of enlargement in (a′, b′). SMAD2P nuclear staining was seen in control (a, a′) and DHT (b, b′) treated E18 lungs in mesenchymal cells (long arrows) and epithelial cells (short arrows). However, epithelial (short arrows) and mesenchymal (long arrows) SMAD2P appeared less evident in DHT-treated lungs (b, b′). In control (c), SMAD7 was seen mostly in clusters of mesenchymal cells at the periphery of the lung (long arrow in (c)) and also in bronchiolar epithelial cells (short arrow in (c)). In DHT-treated lungs (d) the mesenchymal staining for SMAD7 (long arrows in (d)) was more diffuse, whereas bronchiolar epithelial cell (short arrows in (d)) staining was similar to controls. Figures are representative tissue sections from 3 lungs at each condition from 3 experiments; 40x Mag.
