About this Journal Submit a Manuscript Table of Contents
BioMed Research International
Volume 2013 (2013), Article ID 327620, 8 pages
http://dx.doi.org/10.1155/2013/327620
Research Article

A Two-Tube Multiplex Reverse Transcription PCR Assay for Simultaneous Detection of Sixteen Human Respiratory Virus Types/Subtypes

1Key Laboratory for Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, No. 155, Changbai Road, Changping District, Beijing 102206, China
2National Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China
3Hebei Center for Disease Control and Prevention, No. 241, Qingyuan Street, Yuhua District, Shijiazhuang 050000, China

Received 7 April 2013; Accepted 25 June 2013

Academic Editor: Gregory Tannock

Copyright © 2013 Jin Li et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. M. T. Coiras, J. C. Aguilar, M. L. García, I. Casas, and P. Pérez-Breña, “Simultaneous detection of fourteen respiratory viruses in clinical specimens by two multiplex reverse transcription nested-PCR assays,” Journal of Medical Virology, vol. 72, no. 3, pp. 484–495, 2004. View at Publisher · View at Google Scholar · View at Scopus
  2. A. S. Fauci, N. A. Touchette, and G. K. Folkers, “Emerging infectious diseases: a 10-year perspective from the National Institute of Allergy and Infectious Diseases,” Emerging Infectious Diseases, vol. 11, no. 4, pp. 519–525, 2005. View at Scopus
  3. J. Mahony, S. Chong, F. Merante et al., “Development of a respiratory virus panel test for detection of twenty human respiratory viruses by use of multiplex PCR and a fluid microbead-based assay,” Journal of Clinical Microbiology, vol. 45, no. 9, pp. 2965–2970, 2007. View at Publisher · View at Google Scholar · View at Scopus
  4. F. G. Hayden, A. D. M. E. Osterhaus, J. J. Treanor et al., “Efficacy and safety of the neuraminidase inhibitor zanamivir in the treatment of influenzavirus infections,” The New England Journal of Medicine, vol. 337, no. 13, pp. 874–880, 1997. View at Publisher · View at Google Scholar · View at Scopus
  5. P. C. Y. Woo, S. S. Chiu, W. Seto, and M. Peiris, “Cost-effectiveness of rapid diagnosis of viral respiratory tract infections in pediatric patients,” Journal of Clinical Microbiology, vol. 35, no. 6, pp. 1579–1581, 1997. View at Scopus
  6. J. Barenfanger, C. Drake, N. Leon, T. Mueller, and T. Troutt, “Clinical and financial benefits of rapid detection of respiratory viruses: an outcomes study,” Journal of Clinical Microbiology, vol. 38, no. 8, pp. 2824–2828, 2000. View at Scopus
  7. K. J. Henrickson, D. M. E. Osterhaus, and J. A. McCullers, “Advances in the laboratory diagnosis of viral respiratory disease,” Pediatric Infectious Disease Journal, vol. 23, no. 1, pp. S6–S10, 2004. View at Scopus
  8. G. A. Storch, “Diagnostic virology,” Clinical Infectious Diseases, vol. 31, no. 3, pp. 739–751, 2000. View at Publisher · View at Google Scholar · View at Scopus
  9. F. Raymond, J. Carbonneau, N. Boucher, et al., “Comparison of automated microarray detection with real-time PCR assays for detection of respiratory viruses in specimens obtained from children,” Journal of Clinical Microbiology, vol. 47, pp. 743–750, 2009.
  10. N. Krunic, T. D. Yager, D. Himsworth, F. Merante, S. Yaghoubian, and R. Janeczko, “xTAG RVP assay: analytical and clinical performance,” Journal of Clinical Virology, vol. 40, supplement 1, pp. S39–S46, 2007. View at Publisher · View at Google Scholar · View at Scopus
  11. K. Pabbaraju, K. L. Tokaryk, S. Wong, and J. D. Fox, “Comparison of the luminex xTAG respiratory viral panel with in-house nucleic acid amplification tests for diagnosis of respiratory virus infections,” Journal of Clinical Microbiology, vol. 46, no. 9, pp. 3056–3062, 2008. View at Publisher · View at Google Scholar · View at Scopus
  12. F. S. Nolte, D. J. Marshall, C. Rasberry et al., “MultiCode-PLx system for multiplexed detection of seventeen respiratory viruses,” Journal of Clinical Microbiology, vol. 45, no. 9, pp. 2779–2786, 2007. View at Publisher · View at Google Scholar · View at Scopus
  13. H. Li, M. A. McCormac, R. W. Estes et al., “Simultaneous detection and high-throughput identification of a panel of RNA viruses causing respiratory tract infections,” Journal of Clinical Microbiology, vol. 45, no. 7, pp. 2105–2109, 2007. View at Publisher · View at Google Scholar · View at Scopus
  14. M. Qin, D. Wang, F. Huang et al., “Detection of pandemic influenza A H1N1 virus by multiplex reverse transcription-PCR with a GeXP analyzer,” Journal of Virological Methods, vol. 168, no. 1-2, pp. 255–258, 2010. View at Publisher · View at Google Scholar · View at Scopus
  15. X. Hu, Y. Zhang, X. Zhou et al., “Simultaneously typing nine serotypes of enteroviruses associated with hand, foot, and mouth disease by a GeXP analyzer-based multiplex reverse transcription-PCR assay,” Journal of Clinical Microbiology, vol. 50, no. 2, pp. 288–293, 2012. View at Publisher · View at Google Scholar · View at Scopus
  16. J. Li, N. Y. Mao, C. Zhang, et al., “The development of a GeXP-based multiplex reverse transcription-PCR assay for simultaneous detection of sixteen human respiratory virus types/subtypes,” BMC Infectious Diseases, vol. 12, article 189, 2012.
  17. T. Tabone, D. E. Mather, and M. J. Hayden, “Temperature Switch PCR (TSP): robust assay design for reliable amplification and genotyping of SNPs,” BMC Genomics, vol. 10, article 580, 2009. View at Publisher · View at Google Scholar · View at Scopus
  18. Z. Xu, W. Cheng, B. Li, J. Li, B. Lan, and Z. Duan, “Development of a real-time PCR assay for detecting and quantifying human bocavirus 2,” Journal of Clinical Microbiology, vol. 49, no. 4, pp. 1537–1541, 2011. View at Publisher · View at Google Scholar · View at Scopus
  19. L. J. Cui, C. Zhang, T. Zhang, et al., “Human coronaviruses HCoV-NL63 and HCoV-HKU1 in hospitalized children with acute respiratory infections in Beijing, China,” Adv Virol, vol. 2011, Article ID 129134, 6 pages, 2011. View at Publisher · View at Google Scholar
  20. F. Merante, S. Yaghoubian, and R. Janeczko, “Principles of the xTAG respiratory viral panel assay (RVP Assay),” Journal of Clinical Virology, vol. 40, supplement 1, pp. S31–S35, 2007. View at Publisher · View at Google Scholar · View at Scopus
  21. D. F. Bibby, I. Mcelarney, J. Breuer, and D. A. Clark, “Comparative evaluation of the seegene seeplex RV15 and real-time PCR for respiratory virus detection,” Journal of Medical Virology, vol. 83, no. 8, pp. 1469–1475, 2011. View at Publisher · View at Google Scholar · View at Scopus
  22. L. E. S. Bruijnesteijn van Coppenraet, C. M. A. Swanink, A. A. van Zwet et al., “Comparison of two commercial molecular assays for simultaneous detection of respiratory viruses in clinical samples using two automatic electrophoresis detection systems,” Journal of Virological Methods, vol. 169, no. 1, pp. 188–192, 2010. View at Publisher · View at Google Scholar · View at Scopus
  23. H. K. Kim, S. H. Oh, K. A. Yun, H. Sung, and M. N. Kim, “Comparison of anyplex II RV16 with xTAG respiratory viral panel and seeplex RV15 for the detection of respiratory viruses,” Journal of Clinical Microbiology, vol. 51, no. 4, pp. 1137–1141, 2013.
  24. L. Bonzel, T. Tenenbaum, H. Schroten, O. Schildgen, S. Schweitzer-Krantz, and O. Adams, “Frequent detection of viral coinfection in children hospitalized with acute respiratory tract infection using a real-time polymerase chain reaction,” Pediatric Infectious Disease Journal, vol. 27, no. 7, pp. 589–594, 2008. View at Publisher · View at Google Scholar · View at Scopus
  25. G. Cilla, E. Oñate, E. G. Perez-Yarza, M. Montes, D. Vicente, and E. Perez-Trallero, “Viruses in community-acquired pneumonia in children aged less than 3 years old: high rate of viral coinfection,” Journal of Medical Virology, vol. 80, no. 10, pp. 1843–1849, 2008. View at Publisher · View at Google Scholar · View at Scopus
  26. N. J. Gadsby, A. Hardie, E. C. J. Claas, and K. E. Templeton, “Comparison of the Luminex respiratory virus panel fast assay with in-house real-time PCR for respiratory viral infection diagnosis,” Journal of Clinical Microbiology, vol. 48, no. 6, pp. 2213–2216, 2010. View at Publisher · View at Google Scholar · View at Scopus
  27. C. Calvo, M. L. García-García, C. Blanco et al., “Multiple simultaneous viral infections in infants with acute respiratory tract infections in Spain,” Journal of Clinical Virology, vol. 42, no. 3, pp. 268–272, 2008. View at Publisher · View at Google Scholar · View at Scopus
  28. R. Brittain-Long, J. Westin, S. Olofsson, M. Lindh, and L. Andersson, “Prospective evaluation of a novel multiplex real-time PCR assay for detection of fifteen respiratory pathogens-Duration of symptoms significantly affects detection rate,” Journal of Clinical Virology, vol. 47, no. 3, pp. 263–267, 2010. View at Publisher · View at Google Scholar · View at Scopus
  29. K. H. Rand, H. Rampersaud, and H. J. Houck, “Comparison of two multiplex methods for detection of respiratory viruses: filmarray RP and xTAG RVP,” Journal of Clinical Microbiology, vol. 49, no. 7, pp. 2449–2453, 2011. View at Publisher · View at Google Scholar · View at Scopus