A Two-Tube Multiplex Reverse Transcription PCR Assay for Simultaneous Detection of Sixteen Human Respiratory Virus Types/Subtypes
Table 4
Performance of the two-tube assay for individual target compared with the confirmed results.
Viruses
No. of specimens by two-tube/confirmed results
Performance of the two-tube assay compared with confirmed results
+/+
+/−
−/+
−/−
Sensitivity %
Specificity %
PPV %
NPV %
Accordance rate%
Kappa
FluA
20
0
0
227
100
100
100
100
100
1.000
sH1N1
14
0
2
231
87.50
100
100
99.14
99.19
0.929
FluB
1
0
0
246
100
100
100
100
100
1.000
PIV1
1
0
0
246
100
100
100
100
100
1.000
PIV2
2
0
0
245
100
100
100
100
100
1.000
PIV3
24
0
0
223
100
100
100
100
100
1.000
HRV
110
0
3
134
97.35
100
100
97.81
98.79
0.975
HMPV
28
0
0
219
100
100
100
100
100
1.000
Adv
35
0
1
211
97.22
100
100
99.53
99.60
0.984
CoV NL63
1
0
0
246
100
100
100
100
100
1.000
CoV OC43
11
0
0
236
100
100
100
100
100
1.000
CoV 229E
2
0
0
245
100
100
100
100
100
1.000
CoV HKU1
3
0
0
244
100
100
100
100
100
1.000
RSVA
8
0
0
239
100
100
100
100
100
1.000
RSVB
96
0
0
151
100
100
100
100
100
1.000
HBoV
28
0
0
219
100
100
100
100
100
1.000
This table shows the sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and the kappa values for each target using the confirmed results as the reference for comparison. All the specificity and PPV were 100%, all the accordance rate values were above 98.79%, and all the kappa values were above 0.75.
