Table 4: Performance of the two-tube assay for individual target compared with the confirmed results.

Viruses No. of specimens by two-tube/confirmed resultsPerformance of the two-tube assay compared with confirmed results
+/++/−−/+−/−Sensitivity
%
Specificity
%
PPV
%
NPV
%
Accordance rate%Kappa

FluA20002271001001001001001.000
sH1N1140223187.5010010099.1499.190.929
FluB1002461001001001001001.000
PIV11002461001001001001001.000
PIV22002451001001001001001.000
PIV324002231001001001001001.000
HRV1100313497.3510010097.8198.790.975
HMPV28002191001001001001001.000
Adv350121197.2210010099.5399.600.984
CoV NL631002461001001001001001.000
CoV OC4311002361001001001001001.000
CoV 229E2002451001001001001001.000
CoV HKU13002441001001001001001.000
RSVA8002391001001001001001.000
RSVB96001511001001001001001.000
HBoV28002191001001001001001.000

This table shows the sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and the kappa values for each target using the confirmed results as the reference for comparison. All the specificity and PPV were 100%, all the accordance rate values were above 98.79%, and all the kappa values were above 0.75.