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BioMed Research International
Volume 2013 (2013), Article ID 341387, 11 pages
http://dx.doi.org/10.1155/2013/341387
Research Article

A Mouse Model of Interstitial Pneumonitis Induced by Murine Cytomegalovirus Infection after Allogeneic Skin Transplantation

1Department of Microbiology, Anhui Medical University, Hefei, Anhui 230032, China
2Key Laboratory of Gene Resource Utilization for Severe Diseases, The Ministry of Education of China and Anhui Province, Hefei, Anhui 230032, China
3Department of Organ Transplantation, First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, Guangdong 510080, China
4Department of Pathology & Cell Biology, Columbia University, New York, NY 10032, USA

Received 11 April 2013; Revised 4 June 2013; Accepted 14 June 2013

Academic Editor: Michel Mittelbronn

Copyright © 2013 Dequn Ni et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We investigated the effect of murine cytomegalovirus (MCMV) on interstitial pneumonia in transplant recipients in an experimental skin allograft model. Skin transplantation between C57BL/6J and BALB/c mice was performed in the presence or absence of cyclosporin A treatment. Flow cytometry showed that the number of CD4+ and CD8+ cells and the level of IFN-γ decreased significantly in the groups treated with cyclosporin A. We either mock-infected or infected the mice with MCMV by intranasal administration and monitored pathophysiological behavior and body weight. The infected mice were sacrificed at different days postinfection for histology, immunohistochemistry, and molecular biological evaluations. Interstitial pneumonitis was observed in positive control groups as well as in experimental group that received cyclosporin A, a skin transplant, and infected with the highest dose of virus (105 PFU). Transmission electronic microscopy demonstrated the presence of herpes virus particles. MCMV DNA and glycoprotein B were demonstrated in the epithelial cells of the lung tissue in those animals by in situ hybridization and immunohistochemistry, respectively. Our data demonstrated the establishment of a mouse model of interstitial pneumonitis via MCMV infection after allogeneic skin transplantation.