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Figure 7: (a)–(c) H&E staining of lung tissue from infected or mock-infected mice. (a) Tissue from Group E (the mock-infected group) showed the normal histology of the mouse lung. (b)-(c) Tissues from Group D (b) 14 or (c) 21 days postinfection show mononuclear cell infiltration and edema of alveolar epithelia (arrows). (d)–(f) In situ hybridization with MCMV-specific probes showed that MCMV DNA was present in epithelial cells (arrows) of the alveoli in the lung tissue of mice (e) 14 or (f) 21 days postinfection, but not in (d) mock-infected mice. (g)–(i) Immunohistochemical staining using anti-MCMV gB monoclonal antibodies revealed viral protein gB (yellow-brown color, indicated by arrows) appeared in the alveolar epithelia (h) 14 and (i) 21 days postinfection. (g) We observed no staining in the lung tissue of mock-infected mice. Bar = 50 μm.