Table 1: Primers and probes for PCR/RT-PCT, real-time PCR, and in situ hybridization.

Primer namePrimer or probe sequencesPCR product size and reference

IEA Dig probe5′-TTCTCTGTCAGCTAGCCAATGATATCTTCGAGC-3′ [17]

IEA primersForward: 5′-TACAGGACAACAGAACGCTC-3′ 
Reverse: 5′-CCTCGAGTCTGGAACCGAAA-3′
300 bp
[17]

M55 primersForward: 5′-GCGACATACACTTCTCCATT-3′,
Reverse: 5′-CAGAATACGTGGCTCACA-3′,
209 bp

IEB primers and probeForward: 5′-TGCCATACTGCCAGCTGAGA-3′ 
Reverse: 5′-GGCTTCATGATCCACCCTGTT-3′ 
Probe (FAM): 5′-CTGGCATCCAGGAAAGGCTTGGTG-3′
66 bp
[18]

Mouse -actin primers and probe Forward: 5′-GAAGATCAAGATCATTGCTCCT-3′ 
Reverse: 5′-TACTCCTGCTTGCTGATCCA-3′ 
Probe (FAM): 5′-CTGTCCACCTTCCAGCAGA-3′ 
111 bp
[18]