A Mouse Model of Interstitial Pneumonitis Induced by Murine Cytomegalovirus Infection after Allogeneic Skin Transplantation

Table 1

Primers and probes for PCR/RT-PCT, real-time PCR, and in situ hybridization.

Primer name	Primer or probe sequences	PCR product size and reference
IEA Dig probe	5′-TTCTCTGTCAGCTAGCCAATGATATCTTCGAGC-3′	[17]
IEA primers	Forward: 5′-TACAGGACAACAGAACGCTC-3′; Reverse: 5′-CCTCGAGTCTGGAACCGAAA-3′	300 bp; [17]
M55 primers	Forward: 5′-GCGACATACACTTCTCCATT-3′,; Reverse: 5′-CAGAATACGTGGCTCACA-3′,	209 bp
IEB primers and probe	Forward: 5′-TGCCATACTGCCAGCTGAGA-3′; Reverse: 5′-GGCTTCATGATCCACCCTGTT-3′; Probe (FAM): 5′-CTGGCATCCAGGAAAGGCTTGGTG-3′	66 bp; [18]
Mouse 𝛽-actin primers and probe	Forward: 5′-GAAGATCAAGATCATTGCTCCT-3′; Reverse: 5′-TACTCCTGCTTGCTGATCCA-3′; Probe (FAM): 5′-CTGTCCACCTTCCAGCAGA-3′	111 bp; [18]