High-Level Expression of Functionally Active Dengue-2 Non-Structural Antigen 1 Production in Escherichia coli
Figure 2
SDS-PAGE analysis of protein extracted in different time intervals from E. coli cells after being transformed with plasmid pBAD/Thio-NS1 and induced with 0.2% arabinose at 30°C. Lane M: protein marker and Lanes 1–4: protein extracts from different time intervals of 1, 2, 3, 4, and 5 h, respectively (a). The expressed protein in E. coli (after the EK cleavage) has been recognized by mouse monoclonal antibodies raised against dengue NS1 in western blot (b).
