Evaluation of In Vitro Cytoxicity and Genotoxicity of Size-Fractionated Air Particles Sampled during Road Tunnel Construction

Table 2

Nuclear division index ± SD in A549 pulmonary cells treated for 24 hours with airborne extracts of tunnel under construction in an area of Central Italy (positive control EMS 2.4 mM value: NDI = 1.67 ± 0.07).

Tunnel	PM (NDI ± SD)
	A: ø > 2.5 μm	B: 1.0 < ø < 2.5 μm	C: 0.5 < ø < 1.0 μm	D: 0.25 < ø < 0.50 μm	AF: ø < 0.25 μm	Untreated
1	1.82±0.04	1.79±0.04	1.86±0.05	1.87±0.03	1.84±0.04	1.80±0.07
2	1.70±0.06	1.68±0.06	1.70±0.03	1.72±0.07	1.82±0.05	1.73±0.14
3	1.78±0.08	1.85±0.08	1.80±0.05	1.87±0.05	1.81±0.08	1.73±0.14
4	1.73±0.10	1.69±0.12	1.65±0.16	1.69±0.07	1.78±0.05	1.75±0.10
5	1.73±0.07	1.72±0.07	1.73±0.13	1.65±0.06	1.72±0.07	1.75±0.10
6	1.66±0.08	1.72±0.10	1.70±0.06	1.80±0.05	1.71±0.014	1.71±0.12
7	1.74±0.17	1.73±0.08	1.75±0.14	1.71±0.11	1.62±0.07	1.71±0.12