Rapid Nongenomic Action of Aldosterone on Protein Expressions of Hsp90(α and β) and pc-Src in Rat Kidney

Table 1

Median staining intensity (score) of renal Hsp90α, Hsp90β, and pc-Src protein expressions.

	Median staining intensity (score)
	Hsp90𝛼		Hsp90𝛽		pc-Src
	sham	Aldo	sham	Aldo	sham	Aldo
Cortex
Glomerulus	2	2	3	4	1	2
PCT	2	3	2	3	0	3
DCT	1	1	1	2	1	0
CCD	3	2	1	3	2	1
Pcap	1	2	3	1	0	0
Outer medulla
Outer stripe
TALH	3	3	4	4	2	1
MCD	2	2	4	4	2	1
PTs	2	1	2	3	0	0
Inner stripe
TALH	3	4	3	3	1	0
MCD	3	3	3	3	1	0
VR	2	3	4	3	3	4
tLH	1	2	2	2	1	4
Inner medulla
MCD	4	3	4	3	2	1
VR	2	3	2	2	4	4
tLH	2	2	2	3	2	2

Staining intensity: 0: negative, no reactivity; 1: trace, faint, or pale brown staining with less membrane reactivity; 2: weak, light brown staining with incomplete membrane reactivity; 3: moderate, shaded of brown staining of intermediate darkness with usually almost complete membrane reactivity; 4: strong, dark brown to black staining with usually complete membrane pattern, producing a thick outline of the cell [5].
PCT: proximal convoluted tubule; DCT: distal convoluted tubule; CCD: cortical collecting duct; Pcap: peritubular capillary; TALH: thick ascending limb of Henle’s loop; MCD: medullary collecting duct; PTs: proximal straight tubule; VR: vasa recta; tLH: thin limb of Henle’s loop.