Identification of Opportunistic Pathogenic Bacteria in Drinking Water Samples of Different Rural Health Centers and Their Clinical Impacts on Humans

Table 2

Identification of the 55 bacterial strains isolated from the drinking water samples collected from different government hospitals, Mahabubnagar, based on BLAST analysis of the 16S rRNA gene sequences.

Sl no.	Strain no.	Nearest phylogenetic neighbor	16S rRNA gene sequence similarity %
		Gram-negative bacterial strains
		Proteobacteria
		Alphaproteobacteria
1	D24	Methylobacterium chloromethanicum CM4^T CP001298	99.6
2	AL1	Methylobacterium zatmanii DSM 5688^T AB175647	99.7
		Betaproteobacteria
3	AP1	Cupriavidus pauculus LMG 3413^T AF085226	100.0
4	AL3	Delftia lacustris DSM 21246^T EU888308	100.0
		Gammaproteobacteria
5	JD2	Acinetobacter calcoaceticus DSM 30006^T X81661	98.5
6	KL1	Acinetobacter calcoaceticus DSM 30006^T X81661	98.5
7	KL5	Acinetobacter calcoaceticus DSM 30006^T X81661	98.5
8	MB1	Acinetobacter calcoaceticus DSM 30006^T X81661	98.4
9	MK4	Acinetobacter calcoaceticus DSM 30006^T X81661	98.5
10	NR4	Acinetobacter calcoaceticus DSM 30006^T X81661	98.4
11	GD5	Acinetobacter johnsonii DSM 6963^T X81663	99.9
12	NG1	Acinetobacter johnsonii DSM 6963^T X81663	99.5
13	AP2	Acinetobacter junii LMG 998^T AM410704	100.0
14	AP4	Acinetobacter junii LMG 998^T AM410704	100.0
15	GD2	Acinetobacter junii LMG 998^T AM410704	100.0
16	JD4	Acinetobacter junii LMG 998^T AM410704	100.0
17	KL3	Acinetobacter junii LMG 998^T AM410704	100.0
18	KL4	Acinetobacter junii LMG 998^T AM410704	100.0
19	MB3	Acinetobacter junii LMG 998^T AM410704	100.0
20	R1	Acinetobacter junii LMG 998^T AM410704	99.9
21	WN1	Acinetobacter junii LMG 998^T AM410704	99.9
22	WN3	Acinetobacter junii LMG 998^T AM410704	100.0
23	WN4	Acinetobacter junii LMG 998^T AM410704	100.0
24	WN5	Acinetobacter junii LMG 998^T AM410704	99.9
25	MB4	Acinetobacter ursingii LUH 3792^T AJ275038	99.6
26	JD1	Aeromonas aquariorum MDC47^T EU085557	100.0
27	R31	Aeromonas aquariorum MDC47^T EU085557	100.0
28	R30	Aeromonas aquariorum MDC47^T EU085557	99.9
29	GD4	Aeromonas hydrophila subsp. hydrophila ATCC 7966^T CP000462	100.0
30	JD3	Aeromonas ichthiosmia DSM 6393^T X71120	99.8
31	MK2	Aeromonas ichthiosmia DSM 6393^T X71120	99.8
32	R26	Aeromonas punctata subsp. punctata NCIMB 13016^T X60408	99.2
33	NG2	Citrobacter freundii DSM 30039^T AJ233408	98.3
34	R21	Pantoea anthophila LMG 2558^T EF688010	99.8
35	R22	Pantoea anthophila LMG 2558^T EF688010	99.9
36	NR2	Pseudomonas aeruginosa LMG 1242^T Z76651	99.9
37	R6	Pseudomonas alcaligenes LMG 1224^T Z76653	100.0
38	R7	Pseudomonas alcaligenes LMG 1224^T Z76653	99.8
39	R23	Pseudomonas alcaligenes LMG 1224^T Z76653	99.8
40	D22	Pseudomonas otitidis MCC10330^T AY953147	99.8
41	GD6	Pseudomonas otitidis MCC10330^T AY953147	99.8
42	MB2	Pseudomonas otitidis MCC10330^T AY953147	99.6
43	MK3	Pseudomonas otitidis MCC10330^T AY953147	99.7
		Gram-positive bacterial strains
		Actinobacteria
44	R8	Kocuria rhizophila DSM 11926^T Y16264	100.0
		Firmicutes
		Bacilli
45	D12	Bacillus anthracis ATCC 14578^T AB190217	100.0
46	KL2	Bacillus aryabhattai B8W22^T EF114313	98.7
47	NR3	Bacillus aryabhattai B8W22^T EF114313	99.7
48	R5	Bacillus cereus ATCC 14579^T AE016877	100.0
49	JU1	Bacillus infantis SMC 4352-1^T AY904032	99.5
50	JU3	Bacillus licheniformis ATCC 14580^T CP000002	99.9
51	JU4	Bacillus niabensis 4T19^T AY998119	98.9
52	AL4	Bacillus stratosphericus 41KF2a^T AJ831841	99.9
53	R2	Bacillus stratosphericus 41KF2a^T AJ831841	100.0
54	D25	Exiguobacterium indicum HHS31 ^T AJ846291	99.9
55	JU2	Lysinibacillus fusiformis NBRC 15717^T AB271743	99.8

The accession numbers of the 55 strains are GU566304 and GU566358.