352370.fig.002a
(a)
352370.fig.002b
(b)
Figure 2: Decellularized biomatrix. (a) After nonenzymatic removal of fibroblasts, ECM was observed at phase contrast microscope (A). Its composition was revealed by indirect immunofluorescent staining of representative extracellular matrix proteins (B) collagen IV (red), laminin (green), fibronectin (blue), and tenascin-C (yellow). (b) Electrophoresis of biomatrix, followed by immunoblotting, revealed semiquantitative differences in biomatrix composition.