353270.fig.003a
(a)
353270.fig.003b
(b)
353270.fig.003c
(c)
353270.fig.003d
(d)
Figure 3: Fluorescence emission spectra of PLP-GSAM and PMP-GSAM. To allow direct comparison of the data, the reported spectra are normalized for protein concentration. Spectra were collected in 20 mM Tricine buffer, pH 7.9 solutions and were normalized to a protein concentration of 7 μM. (a) Emission spectra of PLP-GSAM (solid line) and PMP-GSAM (dashed line) upon excitation at 298 nm with 3 nm emission and excitation slits. (b) Emission spectra of PLP-GSAM (solid line) and PMP-GSAM (dashed line) upon excitation at 420 nm with 5 nm emission and excitation slits. (c) Emission spectra of PLP-GSAM upon excitation at 320 nm (solid line), 330 nm (dotted line), and 340 nm (dashed line) with 3 nm emission and excitation slits. (d) Emission spectra of PMP-GSAM upon excitation at 320 nm (solid line), 330 nm (dotted line), and 340 nm (dashed line) with 3 nm emission and excitation slits.