Poly-S-Nitrosated Albumin as a Safe and Effective Multifunctional Antitumor Agent: Characterization, Biochemistry and Possible Future Therapeutic Applications
Figure 2
(a) Mitochondrial transmembrane potential is altered by Poly-SNO-HSA treatment. LY-80 cells were cultured with either phosphate buffered saline (PBS), 50 μM HSA, or 50 μM Poly-SNO-HSA for 2 h, followed by addition of rhodamine 123. Results are for one representative experiment. (b) Activation of caspase-3 after Poly-SNO-HSA treatment. LY-80 cells were incubated with either PBS (0), 100 μM of HSA, HSA-I or Poly-SNO-HSA-R or with different concentrations of Poly-SNO-HSA for 24 h. HSA-I and Poly-SNO-HSA-R represent 2-iminothiolane modified HSA and UV-reduced Poly-SNO-HSA, respectively. Results are means ± SD of three separate experiments. (c) Effect of Poly-SNO-HSA on LDH release (cytotoxicity). LY-80 cells were incubated for the indicated times with 100 μM HSA (open squares) or 100 μM Poly-SNO-HSA (closed circles). (d) Effect of Poly-SNO-HSA on LDH release (cytotoxicity). LY-80 cells were treated for 48 h with various concentrations of HSA (open squares) or Poly-SNO-HSA (closed circles). Results from three separate experiments are presented as means. **, compared with control.
