Growth Arrest on Inhibition of Nonsense-Mediated Decay Is Mediated by Noncoding RNA GAS5
Figure 3
Down-regulation of GAS5 alleviates the inhibitory effects of UPF1 siRNA on cell viability and proliferation. CEM-C7, Jurkat, and BJAB cells were transfected with either control (−)siRNA or UPF1-siRNA1. 24 h after transfection, control cells and cells transfected with UPF1 siRNA were transfected with (−)siRNA or GAS5 siRNAs. Viable cell number (a) and cell proliferation (b) were determined after 72 h. Results are calculated as percentage viable cell number and absorbance relative to controls transfected with (−)siRNA. Data represent means s.e.m. from three independent experiments. * compared to UPF1 siRNA, (−)siRNA1-transfected cells.
