Growth Arrest on Inhibition of Nonsense-Mediated Decay Is Mediated by Noncoding RNA GAS5
Figure 4
Down-regulation of GAS5 alleviates the inhibitory effects of aminoglycoside antibiotics on the colony forming ability of the human T-cell lines Jurkat and CEM-C7 and the B-lymphoblastoid cell line BJAB. CEM-C7, Jurkat, and BJAB cells were transfected with either control (−)siRNA or GAS5 siRNA2. 48 h after transfection, transfected cells were treated with gentamycin (100 μg/mL) (a), G418 (0.5 μg/mL) (b), or G418 (0.3–0.5 μg/mL) on BJAB cells (c) for 72 h. Colony forming ability was then determined by plating in soft agar. Results are calculated as percentage colony numbers relative to controls incubated in the absence of aminoglycoside antibiotics. Means s.e.m. from four independent experiments are shown. * compared to (−)siRNA.
