Figure 2: (a) Representative immunoblots of PKC and PKC hydrophobic site phosphorylation, and PDK phosphorylation, in PE-stimulated ferret aorta samples, by using phosphospecific antibodies. (b)–(d) Quantitation of changes of phosphorylation levels of PKC, PKC, and PDK after stimulating with PE (10 μM) in ferret aorta. (b) Time course of phosphorylation at hydrophobic site of PKC (S657). (c) Time course of phosphorylation at hydrophobic site of PKC (S729). (d) Time course of phosphorylation of PDK (S241). Data are mean ± SE; animals. * and ** compared with control (0 minutes).