Regulation of PKC Autophosphorylation by Calponin in Contractile Vascular Smooth Muscle Tissue

Figure 3

Temporal lag between phosphorylation of known direct PKC substrate and downstream PKC-dependent signaling events in aorta samples. (a) Time course of phosphorylation of known intracellular PKC substrate, 𝛼-Adducin after activating tissue with PE (10 μM). Data are mean ± SE; 𝑛=4–7 animals. **𝑃<0.01 compared with control (0 minute). (b) ERK 1/2 after addition of PE (10 μM). (c) Time course of phosphorylation of caldesmon. Data are mean ± SE; 𝑛=4--7 animals. *𝑃<0.05 and **𝑃<0.01 compared with control (0 minutes).

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