358643.fig.003a
(a)
358643.fig.003b
(b)
358643.fig.003c
(c)
Figure 3: Temporal lag between phosphorylation of known direct PKC substrate and downstream PKC-dependent signaling events in aorta samples. (a) Time course of phosphorylation of known intracellular PKC substrate, -Adducin after activating tissue with PE (10 μM). Data are mean ± SE; –7 animals. ** compared with control (0 minute). (b) ERK 1/2 after addition of PE (10 μM). (c) Time course of phosphorylation of caldesmon. Data are mean ± SE; animals. * and ** compared with control (0 minutes).