Isolation, Characterization, and Transplantation of Cardiac Endothelial Cells
Figure 2
Flow-cytometry analysis of primary isolates of mouse cardiac endothelial cells. After mechanical disaggregation and enzymatic digestion, single cell suspension from murine whole heart (a), atrium (b), and ventricle (c) were incubated with combination of fluorescent-antibody to CD45, Sca-1, CD31, and CD34. Cells were first gated to include only small and low granulated cells from FSC-A and SSC-A dot plot. Hematopoietic cells identified by CD45+ were gated out. Sca-1+/CD31+ were identified as endothelial population. This population was further divided into CD34+ or CD34− endothelial cells.
