Isolation, Characterization, and Transplantation of Cardiac Endothelial Cells

Figure 6

Immunophenotype and functional analysis of cultured FACS sorted cardiac endothelial cells. The upper panel were representative photograph of immunofluorescent staining of cultured FACS sorted cardiac endothelial cells for antiCaveolin-1, anti-eNOS, and anti-vWF followed by anti rabbit IgG Alexa 594. Staining with only secondary anti rabbit IgG Alexa 594 antibody shows minimal background red fluorescence. Left panel shows the monochromic photograph for each antibody. Right panel shows each antibody in red and DAPI in blue. Scale bar = 100 μm. The lower panel were cultured FACS sorted cardiac endothelial cells up took Ac-LDL-rhodamine (red), also stained with DAPI (blue) and cultured FACS sorted cardiac endothelial cells were plated in Matrigel for the formation of capillary-like structures. Photographs were taken at 3 hours (scale bar = 500 μm) and 5.5 hours (scale bar = 100 μm) after incubation.