Isolation, Characterization, and Transplantation of Cardiac Endothelial Cells
Figure 8
Tissue sections of the heart following cardiac endothelial cells intravenous injection. FACS sorted GFP positive EC from atrium were stained with PKH26 (red) before intravenous injection in C57BL6 mice or Rag1 null mice. Heart tissues were harvested at (A) 1 day after injection in C57BL6 mice (), 1 month (), and 2 months () in Rag1 null mice. Heart tissue sections were stained with antiCaveolin-1 followed by secondary Alexa 647 (pink) to visualize endothelial cells from 1 day post injected C57BL6 mice and 1 month post injected Rag1 null mice. In 2 month post injected Rag1 null mice, 0.5 mg/mL biotinylated-WGA was injected intravenously 30 minutes before harvesting heart tissue. Then, heart tissue sections were stained with streptavidin-Alexa 647 (magenta pseudocolor) and also stained with DAPI (blue). Bottom: although PKH-26+ GFP+ cardiac endothelial cells were detected in the heart (top), many donor cells (PKH-26+) were found in the lungs, livers, and spleens 4 weeks after transplant. Scale bar = 100 μm.
