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BioMed Research International
Volume 2013 (2013), Article ID 359412, 18 pages
Isolation, Characterization, and Transplantation of Cardiac Endothelial Cells
1Department of Pathology, Institute for Stem Cell and Regenerative Medicine, School of Medicine, University of Washington,
Seattle, WA 98109, USA
2Department of Clinical Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand
3Department of Oral Biology, School of Dentistry, University of Washington, Seattle, WA 98195, USA
4Department of Anatomy, Faculty of Dentistry, Mahidol University, Bangkok 10400, Thailand
Received 1 May 2013; Accepted 28 July 2013
Academic Editor: Hannes Stockinger
Copyright © 2013 Busadee Pratumvinit et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
- D. Lloyd-Jones, R. Adams, M. Carnethon et al., “Heart disease and stroke statistics—2009 update. A report from the American heart association statistics committee and stroke statistics subcommittee,” Circulation, vol. 119, no. 3, pp. 480–486, 2009.
- V. J. Dzau, E. M. Antman, H. R. Black et al., “The cardiovascular disease continuum validated: clinical evidence of improved patient outcomes—part I: pathophysiology and clinical trial evidence (risk factors through stable coronary artery disease),” Circulation, vol. 114, no. 25, pp. 2850–2870, 2006.
- Q. G. Dong, S. Bernasconi, S. Lostaglio et al., “A general strategy for isolation of endothelial cells from murine tissues: characterization of two endothelial cell lines from the murine lung and subcutaneous sponge implants,” Arteriosclerosis, Thrombosis, and Vascular Biology, vol. 17, no. 8, pp. 1599–1604, 1997.
- S. S. Barbieri and B. B. Weksler, “Tobacco smoke cooperates with interleukin-1β to alter β-catenin trafficking in vascular endothelium resulting in increased permeability and induction of cyclooxygenase-2 expression in vitro and in vivo,” FASEB Journal, vol. 21, no. 8, pp. 1831–1843, 2007.
- D. Ribatti, B. Nico, A. Vacca, L. Roncali, and F. Dammacco, “Endothelial cell heterogeneity and organ specificity,” Journal of Hematotherapy and Stem Cell Research, vol. 11, no. 1, pp. 81–90, 2002.
- S. Nees, A. L. Gerbes, and E. Gerlach, “Isolation, identification, and continuous culture of coronary endothelial cells from guinea pig hearts,” European Journal of Cell Biology, vol. 24, no. 2, pp. 287–297, 1981.
- J. G. Derhaag, A. M. Duijvestijn, J. J. Emeis, W. Engels, and P. J. C. Van Breda Vriesman, “Production and characterization of spontaneous rat heart endothelial cell lines,” Laboratory Investigation, vol. 74, no. 2, pp. 437–451, 1996.
- S. Nistri, L. Mazzetti, P. Failli, and D. Bani, “High-yield method for isolation and culture of endothelial cells from rat coronary blood vessels suitable for analysis of intracellular calcium and nitric oxide biosynthetic pathways,” Biological Procedures Online, vol. 4, no. 1, pp. 32–37, 2002.
- P. Cirillo, P. Golino, M. Ragni, A. Guarino, P. Calabro, and M. Chiarriello, “A simple method for the isolation, cultivation, and characterization of endothelial cells from rabbit coronary circulation,” Thrombosis Research, vol. 96, no. 4, pp. 329–333, 1999.
- C. A. Diglio, P. Grammas, F. Giacomelli, and J. Wiener, “Rat heart-derived endothelial and smooth muscle cell cultures: isolation, cloning and characterization,” Tissue and Cell, vol. 20, no. 4, pp. 477–492, 1988.
- B. Teng, H. R. Ansari, P. J. Oldenburg, J. Schnermann, and S. J. Mustafa, “Isolation and characterization of coronary endothelial and smooth muscle cells from A1 adenosine receptor-knockout mice,” American Journal of Physiology, vol. 290, no. 4, pp. H1713–H1720, 2006.
- M. Grafe, K. Graf, W. Auch-Schwelk, D. Terbeek, H. Hertel, and E. Fleck, “Cultivation and characterization of micro- and macrovascular endothelial cells from the human heart,” European Heart Journal, vol. 14, no. I, pp. 74–81, 1993.
- F. M. Marelli-Berg, E. Peek, E. A. Lidington, H. J. Stauss, and R. I. Lechler, “Isolation of endothelial cells from murine tissue,” Journal of Immunological Methods, vol. 244, no. 1-2, pp. 205–215, 2000.
- R. M. Mcdouall, M. Yacoub, and M. L. Rose, “Isolation, culture, and characterisation of MHC class II-positive microvascular endothelial cells from the human heart,” Microvascular Research, vol. 51, no. 2, pp. 137–152, 1996.
- Y.-C. Lim and F. W. Luscinskas, “Isolation and culture of murine heart and lung endothelial cells for in vitro model systems,” Methods in Molecular Biology, vol. 341, pp. 141–154, 2006.
- X. Gu, L. Cheng, W. L. Chueng et al., “Neovascularization of ischemic myocardium by newly isolated tannins prevents cardiomyocyte apoptosis and improves cardiac function,” Molecular Medicine, vol. 12, no. 11-12, pp. 275–283, 2006.
- Q. He and M. J. Spiro, “Isolation of rat heart endothelial cells and pericytes: evaluation of their role in the formation of extracellular matrix components,” Journal of Molecular and Cellular Cardiology, vol. 27, no. 5, pp. 1173–1183, 1995.
- M. Nishida, W. W. Carley, M. E. Gerritsen, O. Ellingsen, R. A. Kelly, and T. W. Smith, “Isolation and characterization of human and rat cardiac microvascular endothelial cells,” American Journal of Physiology, vol. 264, no. 2, pp. H639–H652, 1993.
- D. P. Via, A. L. Plant, and I. F. Craig, “Metabolism of normal and modified low-density lipoproteins by macrophage cell lines of murine and human origin,” Biochimica et Biophysica Acta, vol. 833, no. 3, pp. 417–428, 1985.
- Y. Okaji, N. H. Tsuno, J. Kitayama et al., “A novel method for isolation of endothelial cells and macrophages from murine tumors based on Ac-LDL uptake and CD16 expression,” Journal of Immunological Methods, vol. 295, no. 1-2, pp. 183–193, 2004.
- B. C. Oxhorn, D. J. Hirzel, and L. L. O. Buxton, “Isolation and characterization of large numbers of endothelial cells for studies of cell signaling,” Microvascular Research, vol. 64, no. 2, pp. 302–315, 2002.
- R. Auerbach, R. Lewis, B. Shinners, L. Kubai, and N. Akhtar, “Angiogenesis assays: a critical overview,” Clinical Chemistry, vol. 49, no. 1, pp. 32–40, 2003.
- H. Holthofer, “Lectin binding sites in kidney. A comparative study of 14 animal species,” Journal of Histochemistry and Cytochemistry, vol. 31, no. 4, pp. 531–537, 1983.
- N. Ieronimakis, G. Balasundaram, and M. Reyes, “Direct isolation, culture and transplant of mouse skeletal muscle derived endothelial cells with angiogenic potential,” PLoS ONE, vol. 3, no. 3, Article ID e0001753, 2008.
- D. A. Ingram, L. E. Mead, H. Tanaka et al., “Identification of a novel hierarchy of endothelial progenitor cells using human peripheral and umbilical cord blood,” Blood, vol. 104, no. 9, pp. 2752–2760, 2004.
- M. Reyes, A. Dudek, B. Jahagirdar, L. Koodie, P. H. Marker, and C. M. Verfaillie, “Origin of endothelial progenitors in human postnatal bone marrow,” Journal of Clinical Investigation, vol. 109, no. 3, pp. 337–346, 2002.
- J. W. Ford, T. H. Welling III, J. C. Stanley, and L. M. Messina, “PKH26 and 125I-PKH95: characterization and efficacy as labels for in vitro and in vivo endothelial cell localization and tracking,” Journal of Surgical Research, vol. 62, no. 1, pp. 23–28, 1996.
- J. Middleton, L. Americh, R. Gayon et al., “A comparative study of endothelial cell markers expressed in chronically inflamed human tissues: MECA-79, Duffy antigen receptor for chemokines, von Willebrand factor, CD31, CD34, CD105 and CD146,” Journal of Pathology, vol. 206, no. 3, pp. 260–268, 2005.
- M. J. Cross and L. Claesson-Welsh, “FGF and VEGF function in angiogenesis: signalling pathways, biological responses and therapeutic inhibition,” Trends in Pharmacological Sciences, vol. 22, no. 4, pp. 201–207, 2001.
- R. L. Kendall and K. A. Thomas, “Inhibition of vascular endothelial cell growth factor activity by an endogenously encoded soluble receptor,” Proceedings of the National Academy of Sciences of the United States of America, vol. 90, no. 22, pp. 10705–10709, 1993.
- C. Birchmeier, W. Birchmeier, E. Gherardi, and G. F. Vande Woude, “Met, metastasis, motility and more,” Nature Reviews Molecular Cell Biology, vol. 4, no. 12, pp. 915–925, 2003.
- A. Ladoux and C. Frelin, “Hypoxia is a strong inducer of vascular endothelial growth factor mRNA expression in the heart,” Biochemical and Biophysical Research Communications, vol. 195, no. 2, pp. 1005–1010, 1993.
- L. I. Jian, L. F. Brown, M. G. Hibberd, J. D. Grossman, J. P. Morgan, and M. Simons, “VEGF, flk-1, and flt-1 expression in a rat myocardial infarction model of angiogenesis,” American Journal of Physiology, vol. 270, no. 5, pp. H1803–H1811, 1996.
- M. C. Puri, J. Rossant, K. Alitalo, A. Bernstein, and J. Partanen, “The receptor tyrosine kinase TIE is required for integrity and survival of vascular endothelial cells,” EMBO Journal, vol. 14, no. 23, pp. 5884–5891, 1995.
- T. N. Sato, Y. Tozawa, U. Deutsch et al., “Distinct roles of the receptor tyrosine kinases Tie-1 and Tie-2 in blood vessel formation,” Nature, vol. 376, no. 6535, pp. 70–74, 1995.
- E. A. Jaffe, L. W. Hoyer, and R. L. Nachman, “Synthesis of Von Willebrand factor by cultured human endothelial cells,” Proceedings of the National Academy of Sciences of the United States of America, vol. 71, no. 5, pp. 1906–1909, 1974.
- J. E. Sadler, “Biochemistry and genetics of von Willebrand factor,” Annual Review of Biochemistry, vol. 67, pp. 395–424, 1998.
- U. M. Vischer, F. R. Herrmann, T. Peyrard, R. Nzietchueng, and A. Benetos, “Plasma von Willebrand factor and arterial aging,” Journal of Thrombosis and Haemostasis, vol. 3, no. 4, pp. 794–795, 2005.
- J. S. Pollock, U. Forstermann, J. A. Mitchell et al., “Purification and characterization of particulate endothelium-derived relaxing factor synthase from cultured and native bovine aortic endothelial cells,” Proceedings of the National Academy of Sciences of the United States of America, vol. 88, no. 23, pp. 10480–10484, 1991.
- O. W. Griffith and D. J. Stuehr, “Nitric oxide synthases: properties and catalytic mechanism,” Annual Review of Physiology, vol. 57, pp. 707–736, 1995.
- F. Breviario, L. Caveda, M. Corada et al., “Functional properties of human vascular endothelial cadherin (7B4/cadherin-5), an endothelium-specific cadherin,” Arteriosclerosis, Thrombosis, and Vascular Biology, vol. 15, no. 8, pp. 1229–1239, 1995.
- P. J. Newman, M. C. Berndt, J. Gorski et al., “PECAM-1 (CD31) cloning and relation to adhesion molecules of the immunoglobulin gene superfamily,” Science, vol. 247, no. 4947, pp. 1219–1222, 1990.
- S. M. Albelda, W. A. Muller, C. A. Buck, and P. J. Newman, “Molecular and cellular properties of PECAM-1 (endoCAM/CD31): a novel vascular cell-cell adhesion molecule,” Journal of Cell Biology, vol. 114, no. 5, pp. 1059–1068, 1991.
- L. Fina, H. V. Molgaard, D. Robertson et al., “Expression of the CD34 gene in vascular endothelial cells,” Blood, vol. 75, no. 12, pp. 2417–2426, 1990.
- B. J. Nickoloff, “The human progenitor cell antigen (CD34) is localized on endothelial cells, dermal dendritic cells, and perifollicular cells in formalin-fixed normal skin, and on proliferating endothelial cells and stromal spindle-shaped cells in Kaposi's sarcoma,” Archives of Dermatology, vol. 127, no. 4, pp. 523–529, 1991.
- L. Daviet and J. L. McGregor, “Vascular biology of CD36: roles of this new adhesion molecule family in different disease states,” Thrombosis and Haemostasis, vol. 78, no. 1, pp. 65–69, 1997.
- I. Shiojima, I. Komuro, T. Oka et al., “Context-dependent transcriptional cooperation mediated by cardiac transcription factors Csx/Nkx-2.5 and GATA-4,” Journal of Biological Chemistry, vol. 274, no. 12, pp. 8231–8239, 1999.
- T. J. Lints, L. M. Parsons, L. Hartley, I. Lyons, and R. P. Harvey, “Nkx-2.5: a novel murine homeobox gene expressed in early heart progenitor cells and their myogenic descendants,” Development, vol. 119, no. 2, pp. 419–431, 1993.
- I. Komuro and S. Izumo, “Csx: a murine homeobox-containing gene specifically expressed in the developing heart,” Proceedings of the National Academy of Sciences of the United States of America, vol. 90, no. 17, pp. 8145–8149, 1993.
- G. Nemer and M. Nemer, “Cooperative interaction between GATA5 and NF-ATc regulates endothelial-endocardial differentiation of cardiogenic cells,” Development, vol. 129, no. 17, pp. 4045–4055, 2002.
- B. A. Teicher and S. P. Fricker, “CXCL12 (SDF-1)/CXCR4 pathway in cancer,” Clinical Cancer Research, vol. 16, no. 11, pp. 2927–2931, 2010.
- M. D'Apuzzo, A. Rolink, M. Loetscher et al., “The chemokine SDF-1, stromal cell-derived factor 1, attracts early stage B cell precursors via the chemokine receptor CXCR4,” European Journal of Immunology, vol. 27, no. 7, pp. 1788–1793, 1997.
- C. Feil and H. G. Augustin, “Endothelial cells differentially express functional CXC-chemokine receptor-4 (CXCR-4/fusin) under the control of autocrine activity and exogenous cytokines,” Biochemical and Biophysical Research Communications, vol. 247, no. 1, pp. 38–45, 1998.
- N. A. Cipriani, O. O. Abidoye, E. Vokes, and R. Salgia, “MET as a target for treatment of chest tumors,” Lung Cancer, vol. 63, no. 2, pp. 169–179, 2009.
- F. Relaix, D. Rocancourt, A. Mansouri, and M. Buckingham, “Divergent functions of murine Pax3 and Pax7 in limb muscle development,” Genes and Development, vol. 18, no. 9, pp. 1088–1105, 2004.
- M. Buckingham and F. Relaix, “The role of Pax genes in the development of tissues and organs: Pax3 and Pax7 regulate muscle progenitor cell functions,” Annual Review of Cell and Developmental Biology, vol. 23, pp. 645–673, 2007.
- K. L. Crossin and L. A. Krushel, “Cellular signaling by neural cell adhesion molecules of the immunoglobulin superfamily,” Developmental Dynamics, vol. 218, pp. 260–279, 2000.
- S. V. Shmelkov, R. St.Clair, D. Lyden, and S. Rafii, “AC133/CD133/Prominin-1,” International Journal of Biochemistry and Cell Biology, vol. 37, no. 4, pp. 715–719, 2005.
- A. Pfenniger, J.-P. Derouette, V. Verma et al., “Gap junction protein Cx37 interacts with endothelial nitric oxide synthase in endothelial cells,” Arteriosclerosis, Thrombosis, and Vascular Biology, vol. 30, no. 4, pp. 827–834, 2010.
- L.-L. Zhu, L.-Y. Wu, D. T. Yew, and M. Fan, “Effects of hypoxia on the proliferation and differentiation of NSCs,” Molecular Neurobiology, vol. 31, no. 1–3, pp. 231–242, 2005.
- S. A. Consigli and J. Joseph-Silverstein, “Immunolocalization of basic fibroblast growth factor during chicken cardiac development,” Journal of Cellular Physiology, vol. 146, no. 3, pp. 379–385, 1991.
- A. Ratajska, R. J. Torry, G. T. Kitten, S. J. Kolker, and R. J. Tomanek, “Modulation of cell migration and vessel formation by vascular endothelial growth factor and basic fibroblast growth factor in cultured embryonic heart,” Developmental Dynamics, vol. 203, no. 4, pp. 399–407, 1995.
- D. Fraga, T. Meulia, and S. Fenster, Current Protocols Essential Laboratory Technique: Chapter 10, 2008.
- B. Millauer, S. Wizigmann-Voos, H. Schnurch et al., “High affinity VEGF binding and developmental expression suggest Flk-1 as a major regulator of vasculogenesis and angiogenesis,” Cell, vol. 72, no. 6, pp. 835–846, 1993.
- P. Nyberg, L. Xie, and R. Kalluri, “Endogenous inhibitors of angiogenesis,” Cancer Research, vol. 65, no. 10, pp. 3967–3979, 2005.
- J. C. Voyta, D. P. Via, C. E. Butterfield, and B. R. Zetter, “Identification and isolation of endothelial cells based on their increased uptake of acetylated-low density lipoprotein,” Journal of Cell Biology, vol. 99, no. 6, pp. 2034–2040, 1984.
- Y. Kubota, H. K. Kleinman, G. R. Martin, and T. J. Lawley, “Role of laminin and basement membrane in the morphological differentiation of human endothelial cells into capillary-like structures,” Journal of Cell Biology, vol. 107, no. 4, pp. 1589–1598, 1988.
- A. Passaniti, R. M. Taylor, R. Pili et al., “A simple, quantitative method for assessing angiogenesis and antiangiogenic agents using reconstituted basement membrane, heparin, and fibroblast growth factor,” Laboratory Investigation, vol. 67, no. 4, pp. 519–528, 1992.
- J. Alroy, V. Goyal, and E. Skutelsky, “Lectin histochemistry of mammalian endothelium,” Histochemistry, vol. 86, no. 6, pp. 603–607, 1987.
- O. Cleaver and D. A. Melton, “Endothelial signaling during development,” Nature Medicine, vol. 9, no. 6, pp. 661–668, 2003.
- S. J. Morrison, P. M. White, C. Zock, and D. J. Anderson, “Prospective identification, isolation by flow cytometry, and in vivo self-renewal of multipotent mammalian neural crest stem cells,” Cell, vol. 96, no. 5, pp. 737–749, 1999.
- C. Holmes and W. L. Stanford, “Concise review: stem cell antigen-1: expression, function, and enigma,” Stem Cells, vol. 25, no. 6, pp. 1339–1347, 2007.
- T. P. Gumley, I. F. C. McKenzie, and M. S. Sandrin, “Tissue expression, structure and function of the murine Ly-6 family of molecules,” Immunology and Cell Biology, vol. 73, no. 4, pp. 277–296, 1995.
- A. Woodfin, M.-B. Voisin, and S. Nourshargh, “PECAM-1: a multi-functional molecule in inflammation and vascular biology,” Arteriosclerosis, Thrombosis, and Vascular Biology, vol. 27, no. 12, pp. 2514–2523, 2007.