Research Article
Isolation, Characterization, and Transplantation of Cardiac Endothelial Cells
Table 1
Primer sequences for RT-PCR and q-RT-PCR.
| | Target gene | Forward primer () | Reverse primer () |
| | RT-PCR primer sequences | | | | AC133 | CCATGCAGGAGGAAGTGCTT | TGCTCCACTACATAGTCAATTTGCT | | c-Met | TTATTCATGGGCCGGCTTAA | TGGTGATCTTCTTTCCTGTGACA | | CD36 | CATCCAAATGAAGATGAGCATAGG | CCATGCCAAGGAGCTTGATT | | CXCR-4 | GCCATGGAACCGATCAGTGT | ACAGGTGCAGCCGGTACTTG | | Pax3 | CGCTGTCTGTGATCGGAACA | TCTGCTCCTGCGCTGCTT | | NCAM | TGCTCGTGTGTCCTCCTTGA | GCTTGGCAGCAACTGACCAT | | Flk-1 | AAAGACAACGAGACCCTGGTAGAA | CAATGACAAGAAGGAGCCAGAA | | Tie-1 | TGTTCGTGGCCTCAATGCTA | TCGGATACACACCAAGGCTAAA | | Tie-2 | GGAACCTGACCTCGGTGCTA | CCCTGAACCTTATACCGGATGA | | vWF | GATGTCCAGCTCCCCTTCCT | AGGCGTTTCCGAAGTCTACCA | | Flt-1 | GGTGAAGATTTGCGACTTTGG | CCGCATGCCTTCCTTCAG | | CD34 | CTTCTGCTCCGAGTGCCATT | GCCAAGACCATCAGCAAACAC | | CD31 | GGACTCACGCTGGTGCTCTAT | TTGGATACGCCATGCACCTT | | CX43 | GAACTCTCCTTTTCCTTTGACTTCA | GTTACAGCGAAAGGCAGACTGTT | | CX37 | CAGCACACCCACCCTGATCT | GCCTGCCTCCAGCACACT | | Nfatc | TCTGGAGAGTCCGAGAATCGA | GTATGGGTAGGAGTAGTTGGACTCGTA | | GATA4 | CCCGGGCTGTCATCTCACTA | GTGCCCCAGCCTTTTACTTTG | | GATA6 | AGCAGGACCCTTCGAAACG | GCGCTTCTGTGGCTTGATG | | eNOS | TGATGGCGAAGCGTGTGA | GGGCCTGACATTTCCATGAG | | VeCad | CCAGCCCTACGAACCTAAAGTG | CACCCCGTTGTCTGAGATGA | | NKx2.5 | CCAAGTGCTCTCCTGCTTTCC | AGGTACCGCTGTTGCTTGAAG | | GAPDH | GGGAAGCCCATCACCATCT | GCCTCACCCCATTTGATGTT | | q-RT-PCR primer sequences | | | | Flk-1 | ACTGCAGTGATTGCCATGTTCT | CCTTCATTGGCCCGCTTAA | | VEGFA | GAGCAGAAGTCCCATGAAGTGAT | CAATCGGACGGCAGTAGCTT | | sFlt-1 | CAGTTGTCTCTTATCATCTCAGTTTATTGTT | TTTGGGAGGAGCGTTTCCT | | CD31 | TGCAGGCATCGGCAAAG | GCATTTCGCACACCTGGAT | | CD34 | CTTCTGCTCCGAGTGCCATT | GCCAAGACCATCAGCAAACAC | | eNOS | TTGTCTGCGGCGATGTCA | GAATTCTCTGCACGGTTTGCA | | Ve-cad | AGCGCAGCATCGGGTACT | TCGGAAGAATTGGCCTCTGT | | vWF | GATGTCCAGCTCCCCTTCCT | AGGCGTTTCCGAAGTCTACCA | | GAPDH | GGGAAGCCCATCACCATCT | GCCTCACCCCATTTGATGTT |
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