361906.fig.003a
(a)
361906.fig.003b
(b)
361906.fig.003c
(c)
361906.fig.003d
(d)
361906.fig.003e
(e)
361906.fig.003f
(f)
361906.fig.003g
(g)
361906.fig.003h
(h)
361906.fig.003i
(i)
361906.fig.003j
(j)
361906.fig.003k
(k)
361906.fig.003l
(l)
361906.fig.003m
(m)
361906.fig.003n
(n)
361906.fig.003o
(o)
361906.fig.003p
(p)
Figure 3: Confocal fluorescence microscopy of the cytoskeleton demonstrating the differentiated cell phenotypes from BMSCs cultured on the various test surfaces after 1 and 3 d of culture. Immunofluorescence staining of anti-F-actin (Green: (a), (e), (i), (m)), DAPI nuclear staining (Blue: (b), (f), (j), (n)), antivinculin (Red: (c), (g), (k), (o)), and merged images ((d), (h), (l), (p)). Scale bar = 50 μm. –CH3-modified substrate for 1 day ((a), (b), (c), (d)) and 3 days ((e), (f), (g), (h)), –COOH-modified substrate for 1 day ((i), (j), (k), (l)) and 3 days ((m), (n), (o), (p)).