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BioMed Research International
Volume 2013 (2013), Article ID 368975, 12 pages
http://dx.doi.org/10.1155/2013/368975
Research Article

Genome-Wide Analysis of Human MicroRNA Stability

1Department of Cell Biology, Stem Cell Research Center, Peking University School of Basic Medical Sciences, 38 Xueyuan Road, Beijing 100191, China
2Department of Integrated Chinese and Western Medicine, Peking University School of Basic Medical Sciences, 38 Xueyuan Road, Beijing 100191, China
3Department of Physiology and Pathophysiology, Peking University School of Basic Medical Sciences, 38 Xueyuan Road, Beijing 100191, China
4MOE Key Lab of Molecular Cardiovascular Science, Peking University, 38 Xueyuan Road, Beijing 100191, China
5Department of Biomedical Informatics, Peking University School of Basic Medical Sciences, 38 Xueyuan Road, Beijing 100191, China

Received 18 July 2013; Accepted 26 August 2013

Academic Editor: Edwin Wang

Copyright © 2013 Yang Li et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Increasing studies have shown that microRNA (miRNA) stability plays important roles in physiology. However, the global picture of miRNA stability remains largely unknown. Here, we had analyzed genome-wide miRNA stability across 10 diverse cell types using miRNA arrays. We found that miRNA stability shows high dynamics and diversity both within individual cells and across cell types. Strikingly, we observed a negative correlation between miRNA stability and miRNA expression level, which is different from current findings on other biological molecules such as proteins and mRNAs that show positive and not negative correlations between stability and expression level. This finding indicates that miRNA has a distinct action mode, which we called “rapid production, rapid turnover; slow production, slow turnover.” This mode further suggests that high expression miRNAs normally degrade fast and may endow the cell with special properties that facilitate cellular status-transition. Moreover, we revealed that the stability of miRNAs is affected by cohorts of factors that include miRNA targets, transcription factors, nucleotide content, evolution, associated disease, and environmental factors. Together, our results provided an extensive description of the global landscape, dynamics, and distinct mode of human miRNA stability, which provide help in investigating their functions in physiology and pathophysiology.