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BioMed Research International
Volume 2013 (2013), Article ID 371429, 8 pages
Research Article

Biosynthesis of Osmoregulated Periplasmic Glucans in Escherichia coli: The Phosphoethanolamine Transferase Is Encoded by opgE

1Unité de Glycobiologie Structurale et Fonctionnelle, UMR CNRS 8576, IFR 147, Université des Sciences et Technologies de Lille, 59655 Villeneuve d’Ascq Cedex, France
2Université Lille Nord de France, Lille, France

Received 27 June 2013; Revised 30 August 2013; Accepted 2 September 2013

Academic Editor: Yun-Peng Chao

Copyright © 2013 Sébastien Bontemps-Gallo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. J. Vieira and J. Messing, “The pUC plasmids, and M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers,” Gene, vol. 19, no. 3, pp. 259–268, 1982. View at Publisher · View at Google Scholar · View at Scopus
  2. J.-M. Lacroix, M. Tempête, B. Menichi, and J.-P. Bohin, “Molecular cloning and expression of a locus (mdoA) implicated in the biosynthesis of membrane-derived oligosaccharides in Escherichia coli,” Molecular Microbiology, vol. 3, no. 9, pp. 1173–1182, 1989. View at Scopus
  3. J.-M. Lacroix, E. Lanfroy, V. Cogez, Y. Lequette, A. Bohin, and J.-P. Bohin, “The mdoC gene of Escherichia coli encodes a membrane protein that is required for succinylation of osmoregulated periplasmic glucans,” Journal of Bacteriology, vol. 181, no. 12, pp. 3626–3631, 1999. View at Scopus
  4. C. J. Yanisch-Perron, J. Vieira, and J. Messing, “Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors,” Gene, vol. 33, no. 1, pp. 103–119, 1985. View at Publisher · View at Google Scholar · View at Scopus
  5. F. Bouchart, G. Boussemart, A. F. Prouvost et al., “The virulence of a Dickeya dadantii 3937 mutant devoid of osmoregulated periplasmic glucans (OPGs) is restored by inactivation of the RcsCD RcsB phosphorelay,” Journal of Bacteriology, vol. 192, no. 13, pp. 3484–3490, 2010. View at Publisher · View at Google Scholar · View at Scopus
  6. K. A. Datsenko and B. L. Wanner, “One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products,” Proceedings of the National Academy of Sciences of the United States of America, vol. 97, no. 12, pp. 6640–6645, 2000. View at Publisher · View at Google Scholar · View at Scopus
  7. W. Ebel, G. J. Vaughn, H. K. Peters III, and J. E. Trempy, “Inactivation of mdoH leads to increased expression of colanic acid capsular polysaccharide in Escherichia coli,” Journal of Bacteriology, vol. 179, no. 21, pp. 6858–6861, 1997. View at Scopus
  8. S. Bontemps-Gallo, E. Madec, J. Dondeyne et al., “Concentration of osmoregulated periplasmic glucans (OPGs) modulates the activation level of the RcsCD RcsB phosphorelay in the phytopathogen bacteria Dickeya dadantii,” Environmental Microbiology, vol. 15, pp. 881–894, 2013.
  9. A. A. Bhagwat, W. Jun, L. Liu et al., “Osmoregulated periplasmic glucans of Salmonella enterica serovar Typhimurium are required for optimal virulence in mice,” Microbiology, vol. 155, no. 1, pp. 229–237, 2009. View at Publisher · View at Google Scholar · View at Scopus
  10. J. P. Bohin and J. M. Lacroix, “Osmoregulation in the periplasm,” in The Periplasm, M. Ehrmann, Ed., pp. 325–341, American Society for Microbiology, Washington, DC, USA, 2006.
  11. J.-M. Lacroix, I. Loubens, M. Tempete, B. Menichi, and J.-P. Bohin, “The mdoA locus of Escherichia coli consists of an operon under osmotic control,” Molecular Microbiology, vol. 5, no. 7, pp. 1745–1753, 1991. View at Scopus
  12. Y. Lequette, E. Lanfroy, V. Cogez, J.-P. Bohin, and J.-M. Lacroix, “Biosynthesis of osmoregulated periplasmic glucans in Escherichia coli: the membrane-bound and the soluble periplasmic phosphoglycerol transferases are encoded by the same gene,” Microbiology, vol. 154, no. 2, pp. 476–483, 2008. View at Publisher · View at Google Scholar · View at Scopus
  13. B. J. Jackson, J.-P. Bohin, and E. P. Kennedy, “Biosynthesis of membrane-derived oligosaccharides: characterization of mdoB mutants defective in phosphoglycerol transferase I activity,” Journal of Bacteriology, vol. 160, no. 3, pp. 976–981, 1984. View at Scopus
  14. J. H. Miller, A Short Course in Bacterial Genetics: A Laboratory Manual and Handbook for Escherichia coli and Related Bacteria, Cold Spring Harbor Laboratory Press, New York, NY, USA, 1992.
  15. M. N. Yap, C. H. Yang, J. D. Barak, C. E. Jahn, and A. O. Charkowski, “The Erwinia chrysanthemi type III secretion system is required for multicellular behavior,” Journal of Bacteriology, vol. 187, no. 2, pp. 639–648, 2005. View at Publisher · View at Google Scholar · View at Scopus
  16. T. Silhavy, M. Berman, and L. Enquist, Experiments with Gene Fusions, Cold Spring Harbor Laboratory Press, New York, NY, USA, 1984.
  17. R. G. Spiro, “Analysis of sugars found in glycoproteins,” Methods in Enzymology, vol. 8, pp. 3–27, 1966. View at Publisher · View at Google Scholar · View at Scopus
  18. E. L. Sonnhammer, G. von Heijne, and A. Krogh, “A hidden Markov model for predicting transmembrane helices in protein sequences,” Proceedings of the International Conference on Intelligent Systems for Molecular Biology, vol. 6, pp. 175–182, 1998. View at Scopus