Table 1: Primary antibodies used for immunofluorescence staining (Immf.) and enzyme-linked immunosorbent assay (ELISA) of markers of adhesion and phenotypic maturation of VSMCs.

Antibody againstDeveloped in, typeCompany, Cat. numberDilutionIncubation

Chicken talinMouse, monoclonalSigmaa
T 3287
Immf.: 1 : 200
ELISA: 1 : 500
Immf.: overnight, 4°C
ELISA: 60 min, RTc
Human vinculinMouse,
monoclonal
Sigmaa
V9131
Immf.: 1 : 200
ELISA: 1 : 400
Immf.: overnight, 4°C
ELISA: 60 min, RTc
Recombinant human paxillinRabbit,
polyclonal
Chemiconb
P1093
Immf.: 1 : 200
ELISA: 1 : 400
Immf.: overnight, 4°C
ELISA: 60 min, RTc
Chicken α-actininRabbit,
polyclonal
Sigmaa
A2543
Immf.: 1 : 200
ELISA: 1 : 500
Immf.: overnight, 4°C
ELISA: 60 min, RTc
Synthetic peptide of α-smooth muscle actinMouse,
monoclonal
Sigmaa
A2547
Immf.: 1 : 200
ELISA: 1 : 400
Immf.: overnight, 4°C
ELISA: 60 min, RTc
Synthetic peptide of β-actinMouse,
monoclonal
Sigmaa
A 5441
Immf.: 1 : 200
ELISA: 1 : 400
Immf.: overnight, 4°C
ELISA: 60 min, RTc
Human smooth muscle SM1 and SM2 myosinMouse,
monoclonal
Sigmaa
M7786
Immf.: 1 : 200
ELISA: 1 : 500
Immf.: overnight, 4°C
ELISA: 60 min, RTc

Sigma, St. Louis, MO, USA; Czech Dealer: Sigma-Aldrich S.R.O., Prague, Czech Republic.
bChemicon International Inc., Temecula, CA, USA; Czech dealer: Scintilla S.R.O., Jihlava, Czech Republic.
cRoom temperature (RT).