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BioMed Research International
Volume 2013 (2013), Article ID 373569, 8 pages
A Sensitive Chemotaxis Assay Using a Novel Microfluidic Device
1Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA 02115, USA
2Department of Hepatobiliary Surgery, Union Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, Hubei, China
3Cardiovascular Division, Brigham and Women's Hospital and Harvard Medical School, Boston, MA 02115, USA
4Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02115, USA
Received 30 April 2013; Accepted 18 July 2013
Academic Editor: Koichiro Wada
Copyright © 2013 Chen Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
- P. Friedl and B. Weigelin, “Interstitial leukocyte migration and immune function,” Nature Immunology, vol. 9, no. 9, pp. 960–969, 2008.
- M. O'Hayre, C. L. Salanga, T. M. Handel, and S. J. Allen, “Chemokines and cancer: migration, intracellular signalling and intercellular communication in the microenvironment,” Biochemical Journal, vol. 409, no. 3, pp. 635–649, 2008.
- J. T. Price and E. W. Thompson, “Mechanisms of tumour invasion and metastasis: emerging targets for therapy,” Expert Opinion on Therapeutic Targets, vol. 6, no. 2, pp. 217–233, 2002.
- A. H. Sprague and R. A. Khalil, “Inflammatory cytokines in vascular dysfunction and vascular disease,” Biochemical Pharmacology, vol. 78, no. 6, pp. 539–552, 2009.
- J. Yang and R. A. Weinberg, “Epithelial-mesenchymal transition: at the crossroads of development and tumor metastasis,” Developmental Cell, vol. 14, no. 6, pp. 818–829, 2008.
- J. C. Yarrow, Z. E. Perlman, N. J. Westwood, and T. J. Mitchison, “A high-throughput cell migration assay using scratch wound healing, a comparison of image-based readout methods,” BMC Biotechnology, vol. 4, article 21, 2004.
- D. J. Sieg, C. R. Hauck, D. Ilic et al., “FAK integrates growth-factor and integrin signals to promote cell migration,” Nature Cell Biology, vol. 2, no. 5, pp. 249–256, 2000.
- Z. Yu, N. E. Willmarth, J. Zhou et al., “microRNA 17/20 inhibits cellular invasion and tumor metastasis in breast cancer by heterotypic signaling,” Proceedings of the National Academy of Sciences of the United States of America, vol. 107, no. 18, pp. 8231–8236, 2010.
- S. Boyden, “The chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leucocytes,” The Journal of Experimental Medicine, vol. 115, pp. 453–466, 1962.
- R. C. Savani, C. Wang, B. Yang et al., “Migration of bovine aortic smooth muscle cells after wounding injury. The role of hyaluronan and RHAMM,” Journal of Clinical Investigation, vol. 95, no. 3, pp. 1158–1168, 1995.
- S. Toetsch, P. Olwell, A. Prina-Mello, and Y. Volkov, “The evolution of chemotaxis assays from static models to physiologically relevant platforms,” Integrative Biology, vol. 1, no. 2, pp. 170–181, 2009.
- J. Atencia, G. A. Cooksey, and L. E. Locascio, “A robust diffusion-based gradient generator for dynamic cell assays,” Lab on a Chip, vol. 12, no. 2, pp. 309–316, 2012.
- S.-Y. Cheng, S. Heilman, M. Wasserman, S. Archer, M. L. Shuler, and M. Wu, “A hydrogel-based microfluidic device for the studies of directed cell migration,” Lab on a Chip, vol. 7, no. 6, pp. 763–769, 2007.
- B. G. Chung, A. Manbachi, W. Saadi, F. Lin, N. L. Jeon, and A. Khademhosseini, “A gradient-generating microfluidic device for cell biology,” Journal of Visualized Experiments, no. 7, article e271, 2007.
- U. Haessler, Y. Kalinin, M. A. Swartz, and M. Wu, “An agarose-based microfluidic platform with a gradient buffer for 3D chemotaxis studies,” Biomedical Microdevices, vol. 11, no. 4, pp. 827–835, 2009.
- A. Shamloo, N. Ma, M.-M. Poo, L. L. Sohn, and S. C. Heilshorn, “Endothelial cell polarization and chemotaxis in a microfluidic device,” Lab on a Chip, vol. 8, no. 8, pp. 1292–1299, 2008.
- O. C. Amadi, M. L. Steinhauser, Y. Nishi et al., “A low resistance microfluidic system for the creation of stable concentration gradients in a defined 3D microenvironment,” Biomedical Microdevices, vol. 12, no. 6, pp. 1027–1041, 2010.
- P. J. Mack, Y. Zhang, S. Chung, V. Vickerman, R. D. Kamm, and G. García-Cardeña, “Biomechanical regulation of endothelium-dependent events critical for adaptive remodeling,” Journal of Biological Chemistry, vol. 284, no. 13, pp. 8412–8420, 2009.
- S. Chung, R. Sudo, P. J. MacK, C.-R. Wan, V. Vickerman, and R. D. Kamm, “Cell migration into scaffolds under co-culture conditions in a microfluidic platform,” Lab on a Chip, vol. 9, no. 2, pp. 269–275, 2009.
- V. Vickerman, J. Blundo, S. Chung, and R. Kamm, “Design, fabrication and implementation of a novel multi-parameter control microfluidic platform for three-dimensional cell culture and real-time imaging,” Lab on a Chip, vol. 8, no. 9, pp. 1468–1477, 2008.
- V. V. Abhyankar, M. W. Toepke, C. L. Cortesio, M. A. Lokuta, A. Huttenlocher, and D. J. Beebe, “A platform for assessing chemotactic migration within a spatiotemporally defined 3D microenvironment,” Lab on a Chip, vol. 8, no. 9, pp. 1507–1515, 2008.
- J. S. Garanich, M. Pahakis, and J. M. Tarbell, “Shear stress inhibits smooth muscle cell migration via nitric oxide-mediated downregulation of matrix metalloproteinase-2 activity,” American Journal of Physiology—Heart and Circulatory Physiology, vol. 288, no. 5, pp. H2244–H2252, 2005.