Direct Effect of Chenodeoxycholic Acid on Differentiation of Mouse Embryonic Stem Cells Cultured under Feeder-Free Culture Conditions
Figure 1
Growth rate and cell viability of CDCA-treated mES cells. Changes in mES cell number after CDCA treatment (a) and after the second round of treatment with 100 M CDCA (b). The effects of 100 M CDCA on the viability of subcultured mES cells after 72 h incubation, measured by the WST-1 assay. Data represent viability as a percentage of the control (1000 U/mL of LIF-treated cells) (c). Data are expressed as the mean SE (). LIF: leukemia inhibitory factor; DMSO: dimethyl sulfoxide; CDCA: chenodeoxycholic acid.
