Direct Effect of Chenodeoxycholic Acid on Differentiation of Mouse Embryonic Stem Cells Cultured under Feeder-Free Culture Conditions
Figure 2
Changes of pluripoent markers in CDCA-treated mES cells. mES cells were cultivated with 50 M, 100 M, or 200 M CDCA for 72 h. Subcultured mES cells were treated with 100 M CDCA for another 72 h. The cells were probed with antibodies against pluripotent stem cell markers, alkaline phosphatase (ALP), Oct4, and Nanog. LIF: leukemia inhibitory factor; DMSO: dimethyl sulfoxide; CDCA: chenodeoxycholic acid