Direct Effect of Chenodeoxycholic Acid on Differentiation of Mouse Embryonic Stem Cells Cultured under Feeder-Free Culture Conditions
Figure 3
Characteristic changes in CDCA-treated mES cells. mES cells were cultivated with 50 M, 100 M, or 200 M CDCA for 72 h. Subcultured mES cells were treated with 100 M CDCA for another 72 h. Lineage-specific markers such as nestin and PAX6, -smooth muscle actin (-SMA), and -fetoprotein (-FP). LIF: leukemia inhibitory factor; DMSO: dimethyl sulfoxide; CDCA: chenodeoxycholic acid.