Direct Effect of Chenodeoxycholic Acid on Differentiation of Mouse Embryonic Stem Cells Cultured under Feeder-Free Culture Conditions
Figure 4
Characterization of CDCA-treated mES cells by RT-PCR analysis. (a) The mRNA expression of pluripotent stem cell markers and lineage specific makers were analyzed. The mES cells were cultivated with 50 M, 100 M, or 200 M CDCA for 72 h. Sub-cultured mES cells were treated with 100 μM CDCA for another 72 h. MEF, mouse embryonic fibroblast, LIF, leukemia inhibitory factor; DMSO, dimethyl sulfoxide; CDCA, chenodeoxycholic acid. (b) Relative semi-quantitation of PCR signals by image analysis.
