Direct Effect of Chenodeoxycholic Acid on Differentiation of Mouse Embryonic Stem Cells Cultured under Feeder-Free Culture Conditions

Table 1

Primer sequences and cycling conditions used for RT-PCR.

Gene	Primer sequence	Annealing temperature	Product size
Oct4	Forward 5′-GAAGCCCTCCCTACAGCAGA-3′	60°C	297 bp
	Reverse 5′-CAGAGCAGTGACGGGAACAG-3′
Nanog	Forward 5′-CCCCACAAGCCTTGGAATTA-3′	60°C	255 bp
	Reverse 5′-CTCAAATCCCAGCAACCACA-3′
Nestin	Forward 5′-TAGAGGTGCAGCAGCTGCAG-3′	60°C	170 bp
	Reverse 5′-AGCGATCTGACTCTGTAGAC-3′
NCAM	Forward 5′-AGATGGTCAGTTGCTGCCAA-3′	60°C	187 bp
	Reverse 5′-AGAAGACGGTGTGTCTGCTT-3′
α-SMA	Forward 5′-ACTGGGACGACATGGAAAAG-3′	60°C	240 bp
	Reverse 5′-CATCTCCAGAGTCCAGCACA-3′
Desmin	Forward 5′-TGACAACCTGATAGACGACC-3′	60°C	180 bp
	Reverse 5′-TTAAGGAACGCGATCTCCTC-3′
α-FP	Forward 5′-TGCACGAAAATGAGTTTGGGA-3′	60°C	159 bp
	Reverse 5′-TTGCAGCCAACACATCGCTA-3′
Albumin	Forward 5′-TGCTGCTGATTTTGTTGAGG-3′	60°C	500 bp
	Reverse 5′-GCTCACTCACTGGGGTCTTC-3′
FXR	Forward 5′-TTGCGACAAGTGACCTCCAC-3′	58°C	653 bp
	Reverse 5′-TGATGGTTGAATGTCCGGAG-3′
GAPDH	Forward 5′-GTCATCATACTTGGCAGGTT-3′	60°C	489 bp
	Reverse 5′-GTCGTGGAGTCTACTGGTGT-3′

NCAM: neural cell adhesion molecule; α-SMA: alpha-smooth muscle actin; α-FP: alpha-fetoprotein; FXR: farnesoid X receptor; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.