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BioMed Research International
Volume 2013 (2013), Article ID 376051, 7 pages
http://dx.doi.org/10.1155/2013/376051
Research Article

The Effect of Chlorpyrifos on Isolated Thoracic Aorta in Rats

1Pharmacology and Toxicology Department, Veterinary Faculty, Kırıkkale University, Yahşihan, 71451 Kırıkkale, Turkey
2Pharmacology and Toxicology Department, Veterinary Faculty, Ankara University, Dışkapı, 06110 Ankara, Turkey
3Pharmacology and Toxicology Department, Veterinary Faculty, Erciyes University, Melikgazi, 38039 Kayseri, Turkey
4Pathology Department, Veterinary Faculty, Kırıkkale University, 71451 Kırıkkale, Turkey
5Pathology Department, Veterinary Faculty, Kyrgyzstan-Turkiye Manas University, 720044 Bishkek, Kyrgyzstan
6Biochemistry Department, Veterinary Faculty, Kırıkkale University, 71451 Kırıkkale, Turkey
7Physiology Department, Veterinary Faculty, Kırıkkale University, 71451 Kırıkkale, Turkey

Received 9 April 2013; Revised 3 June 2013; Accepted 9 June 2013

Academic Editor: Esmaiel Jabbari

Copyright © 2013 Ebru Yıldırım et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study investigated the effect of chlorpyrifos on thoracic aorta and on the level of NO in plasma and aorta. The effect of chlorpyrifos on thoracic aorta in organ bath was determined in 10 rats. Another 45 rats were assigned to 3 groups with 15 rats each: control group 1 received distilled water, control group 2 was given corn oil, and the last group was given 13.5 mg/kg chlorpyrifos dissolved in corn oil every other day for 8 weeks orally. Chlorpyrifos (10−10 M–10−5 M) showed no effect on isolated thoracic aorta. Plasma AChE activity was decreased, while LDH, ALT, GGT, and AST activities were increased in chlorpyrifos group compared to control groups. Plasma NO level was increased in chlorpyrifos group compared to control groups. iNOS expression was present in all groups in the cytoplasm of the endothelia and in the smooth muscle cells of aorta. According to semiquantitative histomorphological analysis, iNOS immunopositive reactions were seen in the decreasing order in chlorpyrifos, control 2, and control 1 groups. eNOS immunopositive reactions were observed in the endothelial cell cytoplasm, rarely in the subintimal layer, and the smooth muscle cells of aorta. There were no differences among the groups in terms of eNOS immunostaining. In conclusion, chlorpyrifos induced NO production in aorta following an increase in NOS expression.